Neil O’Brien-Simpson Melbourne Dental School, The University of Melbourne, Australia
2 protocols

Marc-Antoine Sani University of Melbourne
2 protocols

Eric Reynolds Melbourne Dental School, The University of Melbourne, Australia
1 protocol

Katrina Walsh Melbourne Dental School, The University of Melbourne, Australia
1 protocol

Frances Separovic
  • The University of Melbourne
Research focus
  • Molecular biology
  • 2 Author merit


Ph.D. (part time), School of Physics, University of NSW (1986-1992)

Current position

Head, School of Chemistry, University of Melbourne (2010-present)
Professor, School of Chemistry, University of Melbourne (2005-present)

Publications (selected)

Total 195 papers and 217 conference proceedings

  1. Fernandez, D. I., Le Brun, A. P., Whitwell, T. C., Sani, M. A., James, M. and Separovic, F. (2012). The antimicrobial peptide aurein 1.2 disrupts model membranes via the carpet mechanism. Phys Chem Chem Phys 14(45): 15739-15751.
  2. Sani, M. A., Gehman, J. D. and Separovic, F. (2011). Lipid matrix plays a role in Abeta fibril kinetics and morphology. FEBS Lett 585(5): 749-754.
  3. Burrell, G. L., Dunlop, N. F. and Separovic, F. (2010). Non-Newtonian viscous shear thinning in ionic liquids. Soft Matter 6(9): 2080-2086.
  4. Gehman, J. D., Luc, F., Hall, K., Lee, T. H., Boland, M. P., Pukala, T. L., Bowie, J. H., Aguilar, M. I. and Separovic, F. (2008). Effect of antimicrobial peptides from Australian tree frogs on anionic phospholipid membranes. Biochemistry 47(33): 8557-8565.
  5. Lau, T. L., Ambroggio, E. E., Tew, D. J., Cappai, R., Masters, C. L., Fidelio, G. D., Barnham, K. J. and Separovic, F. (2006). Amyloid-beta peptide disruption of lipid membranes and the effect of metal ions. J Mol Biol 356(3): 759-770.
  6. Anderluh, G., Razpotnik, A., Podlesek, Z., Macek, P., Separovic, F. and Norton, R. S. (2005). Interaction of the eukaryotic pore-forming cytolysin equinatoxin II with model membranes: 19F NMR studies. J Mol Biol 347(1): 27-39.
  7. de Planque, M. R., Rijkers, D. T., Fletcher, J. I., Liskamp, R. M. and Separovic, F. (2004). The alphaM1 segment of the nicotinic acetylcholine receptor exhibits conformational flexibility in a membrane environment. Biochim Biophys Acta 1665(1-2): 40-47.
  8. Bonev, B. B., Lam, Y. H., Anderluh, G., Watts, A., Norton, R. S. and Separovic, F. (2003). Effects of the eukaryotic pore-forming cytolysin Equinatoxin II on lipid membranes and the role of sphingomyelin. Biophys J 84(4): 2382-2392.
  9. Lam, Y. H., Bustami, R., Phan, T., Chan, H. K. and Separovic, F. (2002). A solid-state NMR study of protein mobility in lyophilized protein-sugar powders. J Pharm Sci 91(4): 943-951.
  10. Kishore, A., Herberstein, M., Craig, C. and Separovic, F. (2002). Solid‐state NMR relaxation studies of Australian spider silks. Biopolymers 61(4): 287-297.
2 Protocols published
Bacterial Fluorescent-dextran Diffusion Assay
Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance ...
Dye Release Experiments with Dextran Loaded Vesicles
Authors:  Marc-Antoine Sani, Neil M. O’Brien-Simpson and Frances Separovic, date: 07/20/2014, view: 3473, Q&A: 0
Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously ...