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Coauthors
Samuel Wilson Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
3 protocols

Julie Horton Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
1 protocol

Melike Çağlayan
  • Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
Research focus
  • Biochemistry
Contributions
  • 3 Author merit
  • 3 Reviewer merit

Education

Ph.D, Bogazici University, Turkey, 2010

Current position

Research Fellow, GISBL, NIEHS/NIH, US

Publications

  1. Caglayan, M., Horton, J. K., Dai, D. P., Stefanick, D. F. and Wilson, S. H. (2017). Oxidized nucleotide insertion by pol beta confounds ligation during base excision repair. Nat Commun 8: 14045.
  2. Sassa, A., Caglayan, M., Rodriguez, Y., Beard, W. A., Wilson, S. H., Nohmi, T., Honma, M. and Yasui, M. (2016). Impact of Ribonucleotide Backbone on Translesion Synthesis and Repair of 7,8-Dihydro-8-oxoguanine. J Biol Chem 291(46): 24314-24323.
  3. Caglayan, M. and Wilson, S. H. (2015). Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair. DNA Repair (Amst) 35: 85-89.
  4. Caglayan, M., Horton, J. K., Prasad, R. and Wilson, S. H. (2015). Complementation of aprataxin deficiency by base excision repair enzymes. Nucleic Acids Res 43(4): 2271-2281.
  5. Caglayan, M., Batra, V. K., Sassa, A., Prasad, R. and Wilson, S. H. (2014). Role of polymerase beta in complementing aprataxin deficiency during abasic-site base excision repair. Nat Struct Mol Biol 21(5): 497-499.
  6. Sassa, A., Caglayan, M., Dyrkheeva, N. S., Beard, W. A. and Wilson, S. H. (2014). Base excision repair of tandem modifications in a methylated CpG dinucleotide. J Biol Chem 289(20): 13996-14008.
  7. Caglayan, M. and Bilgin, N. (2012). Temperature dependence of accuracy of DNA polymerase I from Geobacillus anatolicus. Biochimie 94(9): 1968-1973.
  8. Caglayan, M. and Bilgin, N. (2011). Cloning and sequence analysis of novel DNA polymerases from thermophilic Geobacillus species isolated from hot springs in Turkey: characterization of a DNA polymerase I from Geobacillus kaue strain NB. Appl Biochem Biotechnol 165(5-6): 1188-1200.
3 Protocols published
In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
Authors:  Melike Çağlayan and Samuel H. Wilson, date: 06/20/2017, view: 1021, Q&A: 0
We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide ...
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells
Authors:  Melike Çağlayan, Julie K. Horton and Samuel H. Wilson, date: 06/05/2015, view: 2269, Q&A: 0
We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, ...