Search
Coauthors
Xin Li Department of Botany and Michael Smith Laboratories, University of British Columbia, Canada
1 protocol

Charles Copeland Department of Botany and Michael Smith Laboratories, University of British Columbia, Canada
2 protocols
Coreviewers
Arsalan Daudi University of California
137 protocols

Fernanda Salvato North Carolina State University
5 protocols

Gaston Pizzio Centre for Research in Agricultural Genomics
2 protocols

Longping Tse University of North Carolina at Chapel Hill
13 protocols

Reviewer
Fang Xu
  • Post-Doc, Cold Spring Harbor Laboratory
Research focus
  • Plant science
  • maize meristem development
Contributions
  • 2 Author merit
  • 9 Reviewer merit

Education

Ph.D, University of British Columbia, 2014

Lab information

David Jackson's Lab

Publications

Xu F#, Huang Y#, Li L#, Gannon P, Kapos P, Linster E, Bienvenut W, Polevoda B, Wirtz M, Meinnel T, Hell R, Giglione C, Chen S, Zhang Y, and Li X* (2015). Two N-terminal acetyltransferases antagonistically control the stability of a Nod-like receptor in plant. The Plant Cell 27: 1547–1562. # These authors contributed equally.
-The above paper was highlighted in Nature Plants: Immunity: Antagonistic acetylases doi: 10.1038/nplants.2015.84
Xu F#, Zhu C#, Cevik V#, Johnson K, Liu Y, Sohn Kee, Holub E, Jones J, Liu Y, Li X*. (2015). Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TIR-NB-LRR neighbor. Scientific Reports. Doi: 10.1038/srep08792. # These authors contributed equally.
Kapos P#, Xu F#, Giglione C and Li X*. (2015) N-terminal modifications contribute to flowering time and immune response regulations. Plant Signal Behav Sep 11:0. # These authors contributed equally.
Xu F, Kapos P, Li M, Zhang Y, Li X*. (2014) NLR-associating transcription factor bHLH84 and its paralogs function redundantly in plant immunity. PLoS Pathogens 10(8):e1004312
Xu F#, Cheng YT#, Li M, Li X*. (2014) P-loop-dependent NLR SNC1 can oligomerize and activate immunity in the nucleus. Molecular Plant. doi: 10.1093/mp/ssu097. # These authors contributed equally.
Xu F, Xu S, Wiermer M, Zhang Y, Li X*. (2012) The cyclin L homolog MOS12 and the MOS4-associated complex are required for the proper splicing of plant resistance genes. The Plant Journal. 70(6):916-28.
Xu F* and Copeland C. (2012) Nuclear extraction from Arabidopsis thaliana. Bio-Protocol 2(24).
Xu F*, Copeland C and Li X. (2015) Protein Immunoprecipitation Using Nicotiana benthamiana Transient Expression System. Bio-Protocol 5(13)
Xu S, Zhang Z, Jing B, Gannon P, Ding J, Xu F, Li X, Zhang Y*. (2011) Transportin-SR is required for proper splicing of resistance genes and plant immunity. PLoS Genetics 7(6):e1002159.
Zhu Z, Xu F, Zhang Y, Cheng YT, Wiermer M, Li X, Zhang Y*. (2010) Arabidopsis resistance protein SNC1 activates immune responses through association with a transcriptional corepressor. Proc Natl Acad Sci U S A. 107(31):13960-5.
Monaghan J, Xu F, Xu S, Zhang Y, Li X*. (2010) Two putative RNA-binding proteins function with unequal genetic redundancy in the MOS4-associated complex. Plant Physiol. 154(4):1783-93.
Zhang Y, Xu S, Ding P, Wang D, Cheng YT, He J, Gao M, Xu F, Li Y, Zhu Z, Li X, Zhang Y*. (2010) Control of salicylic acid synthesis and systemic acquired resistance by two members of a plant-specific family of transcription factors. Proc Natl Acad Sci U S A. 107(42):18220-5.
Xu F, Monaghan J, Chen S, Zhang Y, and Li X*. (2009) Analysis of MOS4-Associated protein Complex (MAC) by immuno-affinity chromatography and quantitative mass spectrometry. Chapter 84 in Biology of Molecular Plant-Microbe Interactions, Volume 7.
Monaghan J, Xu F, Gao M, Zhao Q, Palma K, Long C, Chen S, Zhang Y, Li X*. (2009) Two Prp19-like U-box proteins in the MOS4-associated complex play redundant roles in plant innate immunity. PLoS Pathogens. 5(7):e1000526.
Cheng YT#, Germain H#, Wiermer M#, Bi D#, Xu F, García AV, Wirthmueller L, Després C, Parker JE, Zhang Y, Li X*. (2009) Nuclear Pore Complex Component MOS7/Nup88 Is Required for Innate Immunity and Nuclear Accumulation of Defense Regulators in Arabidopsis. Plant Cell. 21(8):2503-16. # These authors contributed equally.
Gao M, Wang X, Wang D, Xu F, Ding X, Zhang Z, Bi D, Cheng YT, Chen S, Li X, Zhang Y*. (2009) Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe. 6(1):34-44.

2 Protocols published
Protein Immunoprecipitation Using Nicotiana benthamiana Transient Expression System
Authors:  Fang Xu, Charles Copeland and Xin Li, date: 07/05/2015, view: 5806, Q&A: 0
Nicotiana benthamiana (N. benthamiana) is a useful model system to transiently express protein at high level. This protocol describes in detail how to transiently express protein in N. benthamiana and how to carry out ...
Nuclear Extraction from Arabidopsis thaliana
Authors:  Fang Xu and Charles Copeland, date: 12/20/2012, view: 13263, Q&A: 7
This protocol is to isolate nuclei from Arabidopsis cells. They can be further used for other experiments, such as nuclear protein detection, nuclear protein immunoprecipitation and so on.
9 Protocols reviewed
Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors
Authors:  Chin-kai Chuang, Ching-Fu Tu and Chien-Hong Chen, date: 06/05/2017, view: 1302, Q&A: 0
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription ...
More >
Resin-embedded Thin-section Immunohistochemistry Coupled with Triple Cellular Counterstaining
Authors:  William M Palmer, John W Patrick and Yong-Ling Ruan, date: 04/05/2017, view: 955, Q&A: 0
This protocol was developed to study protein localisation within the vascular bundles of developing tomato fruit however, it can be applied to any resin embedded plant tissue. The vascular bundle is comprised of many different cells that all have ...
More >