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Coauthors
Edgardo Moreno Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Costa Rica
1 protocol

Esteban Chaves-Olarte Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, Costa Rica
1 protocol

Carlos Chacón-Díaz Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, Costa Rica
1 protocol

Reviewer
Elías Barquero-Calvo
  • Research associate, Universidad Nacional
Research focus
  • Microbiology
  • Brucella
    Neutrophils
    Vetrinary
Contributions
  • 1 Author merit
  • 4 Reviewer merit

Education

PhD, University of Costa Rica, 2013

Publications

1. Suárez-Esquivel M, Alfaro-Alarcón A, Guzmán-Verri C, Barquero-Calvo E. 2017. Analysis of the association between Helicobacter spp. burden and gastric lesions in dogs. Am J Vet Res. (In press)

2. Rojas I, Barquero-Calvo E, Rojas N, van Balen J, Muñoz-Vargas L, Hoet A. 2017. High prevalence of multidrug resistant community-acquired methicillin-resistant Staphylococcus aureus at the largest veterinary teaching hospital in Costa Rica. Vector Borne Zoonotic Dis. 17(9):645-653. doi: 10.1089/vbz.2017.2145.

3. Hernández-Mora G., Ruiz- Villalobos N., Bonilla-Montoya R., Romero, Zúñiga JJ, Jiménez-Arias J, González-Barrientos R., Baquero-Calvo E, Chacón Díaz C, Rojas N., Chaves-Olarte E., Guzmán-Verri C, Moreno E. 2017. Epidemiology of bovine brucellosis in Costa Rica: lessons learned from failures in the control of the disease. PLoS One. 12(8):e0182380. doi: 10.1371/journal.pone.0182380.

4. Hernández-Mora G, Bonilla-Montoya Ro, Barrantes-Granados O, Esquivel-Suárez A, Montero-Caballero D, González-Barrientos R, Fallas-Monge Z, Palacios-Alfaro JD, Baldi M, Campos E, Chanto G, Barquero-Calvo E, Chacón-Díaz C, Chaves-Olarte E, Guzmán Verri Ca, Romero-Zúñiga JJ, Moreno E. 2017. Brucellosis in mammals of Costa Rica: an epidemiological survey. PLoS One. 12(8):e0182644. doi: 10.1371/journal.pone.0182644.

5. Suárez-Esquivel M, Baker KS, Ruiz-Villalobos N, Hernández-Mora G, Barquero-Calvo E, González-Barrientosc R, Castillo-Zeledón A, Jiménez-Rojasa C, Chacón-Díaz C, Cloeckaerte A, Chaves-Olarte E, Thomson N R, Moreno E and Guzmán-Verri C. 2017. Brucella genetic variability in wildlife marine mammals populations relates to host preference and ocean distribution. Genome Biol Evol. 9(7):1901-1912. doi: 10.1093/gbe/evx137.
6. Araya-Hidalgo E, Gutiérrez-Jiménez C, Chaves-Ramírez M, Suárez-Esquivel M, Guzmán-Verri C, Barquero-Calvo E. 2017. Sequence analysis of the hypervariable region in hmtp210 of Avibacterium paragallinarum. J Vet Med Sci. 79(7):1210-1214. doi: 10.1292/jvms.16-0530.

7. Suárez-Esquivel M, Ruiz-Villalobos N, Jiménez-Rojas C, Barquero-Calvo E, Chacón-Díaz C, Víquez-Ruiz E, Rojas-Campos N, Baker KS, Oviedo-Sánchez G, Amuy E, Chaves-Olarte E, Thomson NR, Moreno E, Guzmán-Verri C. 2017. Brucella neotomae Infection in Humans, Costa Rica. Emerg Infect Dis. 23(6):997-1000. doi: 10.3201/eid2306.162018.

8. Flores-Díaz M, Barquero-Calvo E, Ramírez M, Alape-Girón A. 2016. Role of Clostridium perfringens Toxins in Necrotic Enteritis in Poultry, p. 1–16. In Gopalakrishnakone, P, Stiles, B, Alape-Girón, A, Dubreuil, JD, Mandal, M (eds.), Microbial Toxins. Springer Netherlands, Dordrecht

9. M Suárez-Esquivel, N Ruiz-Villalobos, A Castillo-Zeledón, C Jiménez-Rojas, M R Roop II, D J. Comerci, E Barquero-Calvo, C Chacón-Díaz, C Caswell, K S Baker, E Chaves-Olarte, N R Thomson, E Moreno, J-J Letesson, X De Bolle, C Guzmán-Verri. 2016. Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains. Front Microbiol. 7:1557.

10. Mora-Cartín, R., C. Chacón-Díaz, C. Gutiérrez-Jiménez, S. Gurdián-Murillo, B. Lomonte, E. Chaves-Olarte, E. Barquero-Calvo, E. Moreno. 2016. N-Formyl-perosamine surface homopolysaccharides hinder the recognition of Brucella abortus by mouse neutrophils. Infect Immun. 84(6):1712-21

11. Barquero-Calvo E. 2015. Estudiando el papel de los neutrófilos polimorfonucleares en la brucelosis. Rev Colegio de Microb Quím Clin Costa Rica. 21(4):103-108

12. Chacón-Díaz C, Altamirano-Silva P, González-Espinoza G, Medina MC, Alfaro-Alarcón A, Bouza-Mora L, Jiménez-Rojas C, Wong M, Barquero-Calvo E, Rojas N, Guzmán-Verri C, Moreno E, Chaves-Olarte E. 2015. Brucella canis is an intracellular pathogen inducing a lower proinflammatory response than smooth zoonotic counterparts. Infect Immun. 83(12):4861-70. doi: 10.1128/IAI.00995-15.

13. Mayorga M, Rodríguez-Cavallini E, López-Ureña D, Barquero-Calvo E, Quesada-Gómez C. 2015. Identification and antimicrobial susceptibility of obligate anaerobic bacteria from clinical samples of animal origin. Anaerobe. 15;36:19-24.

14. Jiménez RR, Barquero-Calvo E, Abarca JG, Porras LP. 2015. Salmonella Isolates in the Introduced Asian House Gecko (Hemidactylus frenatus) with Emphasis on Salmonella Weltevreden, in Two Regions in Costa Rica. Vector Borne Zoonotic Dis. 15(9):550-5.

15. Barquero-Calvo E, Mora-Cartín R, Arce-Gorvel V, de Diego JL, Chacón-Díaz C, et al. 2015. Brucella abortus Induces the Premature Death of Human Neutrophils through the Action of Its Lipopolysaccharide. PLoS Pathog. 11(5): e1004853.

16. Rivera-Pérez W, Barquero-Calvo E, Zamora-Sanabria R. 2014. Salmonella Contamination Risk Points in Broiler Carcasses during Slaughter Line Processing. J Food Prot. 77(12):2031–2034

17. Barquero-Calvo E, Chacón-Díaz C, Chaves-Olarte E and Moreno, E. 2013. Bacterial Counts in Spleen. Bio-protocol 3(21): e954. DOI: 10.21769/BioProtoc.954

18. Barquero-Calvo E, Martirosyan A, Ordoñez-Rueda D, Arce-Gorvel V, Alfaro-Alarcón A, Lepidi H, Malissen B, Malissen M, Gorvel J-P, Moreno E. 2013. Neutrophils exert a suppressive effect on Th1 responses to intracellular pathogen Brucella abortus. PLoS Pathog. 9(2): e1003167.

19. Conde-Álvarez R, Arce-Gorvel V, Iriarte M, Barquero-Calvo E, Palacios-Chaves L, Chacón-Díaz C, Chaves-Olarte E, Martirosyan A, Grilló M-J, Jerala R, Brandenburg K, Llobet E, Bengoechea J-A, Moreno E, Moriyón I, Gorvel J-P. 2012. The Lipopolysaccharide Core of Brucella abortus Acts as a Shield Against Innate Immunity Recognition. PLoS Pathog. (5): e1002675.

20. Guzmán-Verri C, González-Barrientos R, Hernández-Mora G, Morales J-A, Baquero-Calvo E, Chaves-Olarte E and Moreno E. 2012. Brucella ceti and brucellosis in cetaceans. Front Cell Infect Microbiol. 2:3. doi: 10.3389/fcimb.2012.00003.

21. López-Goñi I, García-Yoldi D, Marín CM, de Miguel MJ, Barquero-Calvo E, Guzmán-Verri C, Albert D, Garin-Bastuji B. 2011. New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis. Vet Microbiol. 154(1-2):152-5. doi: 10.1016/j.vetmic.2011.06.035.

22. Chacón-Díaz C, Muñoz-Rodríguez M, Barquero-Calvo E, Guzmán-Verri C, Chaves-Olarte E, Grilló MJ, Moreno E. 2011. The use of green fluorescent protein as a marker for Brucella vaccines. Vaccine. 29(3):577-82. doi: 10.1016/j.vaccine.2010.09.109.

23. Palacios-Chaves L, Conde-Álvarez R, Gil-Ramírez Y, Zúñiga-Ripa A, Barquero-Calvo E, Chacón-Díaz C, Chaves-Olarte E, Arce-Gorvel V, Gorvel JP, Moreno E, de Miguel MJ, Grilló MJ, Moriyón I and Iriarte M. 2011. Brucella abortus Ornithine Lipids Are Dispensable Outer Membrane Components Devoid of a Marked Pathogen-Associated Molecular Pattern. PLoS One. 6(1):e16030. doi: 10.1371/journal.pone.0016030.

24. González-Barrientos R, Morales JA, Hernández-Mora G, Barquero-Calvo E, Guzmán-Verri C, Chaves-Olarte E, Moreno E. 2010. Pathology of Brucella ceti infected Stenella coeruleoalba dolphins. J Comp Pathol. 142(4):347-52. doi: 10.1016/j.jcpa.2009.10.017.

25. Baldi M., J.A. Morales G. Hernández M. Jiménez A. Alfaro, Barquero-Calvo E. 2010. Chromobacterium violaceum infection in a free ranging howler monkey in Costa Rica. J Wildl Dis. 46(1):306-10.

26. Barquero-Calvo E, Conde-Alvarez R, Chacón-Díaz C, Quesada-Lobo L, Martirosyan A, Guzmán-Verri C, Iriarte M, Mancek-Keber M, Jerala R, Gorvel JP, Moriyón I, Moreno E, Chaves-Olarte E. 2009. The Differential Interaction of Brucella and Ochrobactrum with Innate Immunity Reveals Traits Related to the Evolution of Stealthy Pathogens. PLoS One. 4(6):e5893. doi: 10.1371/journal.pone.0005893.

27. Hernández-Mora G, Manire CA, González-Barrientos R, Barquero-Calvo E, Guzmán-Verri C, Staggs L, Thompson R, Chaves-Olarte E, Moreno E. 2009. Serological Diagnosis of Brucella Infections in Odontocetes. Clin Vaccine Immunol. 16(6):906-15. doi: 10.1128/CVI.00413-08.

28. Hernández-Mora G, González-Barrientos R, Morales JA, Chaves-Olarte E, Guzmán-Verri C, Barquero-Calvo E, De-Miguel MJ, Marín CM, Blasco JM, Moreno E. 2008. Neurobrucellosis in stranded dolphins, Costa Rica. Emerg Infect Dis. 14(9):1430-3. doi: 10.3201/eid1409.071056.

29. Manterola L, Guzmán-Verri C, Chaves-Olarte E, Barquero-Calvo E, de Miguel MJ, Moriyón I, Grillo MJ, Lopez-Goni I, Moreno E. 2007. BvrR/BvrS-controlled outer membrane proteins Omp3a and Omp3b are not essential for Brucella abortus virulence. Infect Immun. 75(10):4867-74.

30. Barquero-Calvo E, Chaves-Olarte E, Weiss DS, Guzmán-Verri C, Chacón-Díaz C, Rucavado A, Moriyon I, Moreno E. 2007. Brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. PLoS One. 18;2(7):e631.

1 Protocol published
Bacterial Counts in Spleen
Bacterial loads can be determined as colony forming units (CFU) at any point of the infection by culturing spleen homogenates on agar plates. This is a reliable method for comparing the kinetics of infection in various mouse strains, estimating the ...