Harald Putzer
  • CNRS FRE3630, Institut de Biologie Physico-Chimique, France
  • 2 Author merit

Current position

CNRS Director of Research at the Institut de Biologie Physico-Chimique in Paris, France. His research focuses on RNA metabolism and gene regulation in Gram positive bacteria


  1. Taverniti, V., Forti, F., Ghisotti, D. and Putzer, H. (2011). Mycobacterium smegmatis RNase J is a 5'-3' exo-/endoribonuclease and both RNase J and RNase E are involved in ribosomal RNA maturation. Mol Microbiol 82(5): 1260-1276.
  2. Laalami, S. and Putzer, H. (2011). mRNA degradation and maturation in prokaryotes: the global players. Biomolecular Concepts 2(6): 491-506.
  3. Bruscella, P., Shahbabian, K., Laalami, S. and Putzer, H. (2011). RNase Y is responsible for uncoupling the expression of translation factor IF3 from that of the ribosomal proteins L35 and L20 in Bacillus subtilis. Mol Microbiol 81(6): 1526-1541.
  4. Dorleans, A., Li de la Sierra-Gallay, I., Piton, J., Zig, L., Gilet, L., Putzer, H. and Condon, C. (2011). Molecular basis for the recognition and cleavage of RNA by the bifunctional 5'-3' exo/endoribonuclease RNase J. Structure 19(9): 1252-1261.
  5. Hamze, K., Autret, S., Hinc, K., Laalami, S., Julkowska, D., Briandet, R., Renault, M., Absalon, C., Holland, I. B., Putzer, H. and Seror, S. J. (2011). Single-cell analysis in situ in a Bacillus subtilis swarming community identifies distinct spatially separated subpopulations differentially expressing hag (flagellin), including specialized swarmers. Microbiology 157(Pt 9): 2456-2469.
  6. Brill, J., Hoffmann, T., Putzer, H. and Bremer, E. (2011). T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis. Microbiology 157(Pt 4): 977-987.
  7. Mathy, N., Hebert, A., Mervelet, P., Benard, L., Dorleans, A., Li de la Sierra-Gallay, I., Noirot, P., Putzer, H. and Condon, C. (2010). Bacillus subtilis ribonucleases J1 and J2 form a complex with altered enzyme behaviour. Mol Microbiol 75(2): 489-498.
  8. Shahbabian, K., Jamalli, A., Zig, L. and Putzer, H. (2009). RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilis. EMBO J 28(22): 3523-3533.
  9. Andre, G., Even, S., Putzer, H., Burguiere, P., Croux, C., Danchin, A., Martin-Verstraete, I. and Soutourina, O. (2008). S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum. Nucleic Acids Res 36(18): 5955-5969.
  10. Mader, U., Zig, L., Kretschmer, J., Homuth, G. and Putzer, H. (2008). mRNA processing by RNases J1 and J2 affects Bacillus subtilis gene expression on a global scale. Mol Microbiol 70(1): 183-196.
  11. Li de la Sierra-Gallay, I., Zig, L., Jamalli, A. and Putzer, H. (2008). Structural insights into the dual activity of RNase J. Nat Struct Mol Biol 15(2): 206-212.
  12. Choonee, N., Even, S., Zig, L. and Putzer, H. (2007). Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism. Nucleic Acids Res 35(5): 1578-1588.
  13. Seliverstov, A. V., Putzer, H., Gelfand, M. S. and Lyubetsky, V. A. (2005). Comparative analysis of RNA regulatory elements of amino acid metabolism genes in Actinobacteria. BMC Microbiol 5: 54.
2 Protocols published
Synthesis of 5’ end-labeled RNA
Author:  Harald Putzer, date: 07/20/2012, view: 6338, Q&A: 2
5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases ...
Primer Extension Analysis Using AMV Reverse Transcriptase
Author:  Harald Putzer, date: 07/20/2012, view: 4961, Q&A: 0
Primer extension analysis is a useful method to determine the transcription start point or a processing site on an RNA molecule. It can also allow a quantitative measurement of an RNA species.