Michele Calos Department of Genetics, Stanford University, USA
1 protocol

Jonathan Geisinger
  • Stanford University
Research focus
  • Molecular biology
  • 1 Author merit


Ph.D of Genetics, Department of Genetics, Stanford University, USA, 2015

Current position

Postdoctoral Researcher, Department of Biology, Stanford University, California, USA


  1. Geisinger, J. M., Turan, S., Hernandez, S., Spector, L. P. and Calos, M. P. (2016). In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining. Nucleic Acids Res 44(8): e76.
  2. Geisinger, J. M. and Calos, M. P. (2015). Using phage integrases in a site-specific dual integrase cassette exchange strategy. Methods Mol Biol 1239: 29-38.
  3. Zhao, C., Farruggio, A. P., Bjornson, C. R., Chavez, C. L., Geisinger, J. M., Neal, T. L., Karow, M. and Calos, M. P. (2014). Recombinase-mediated reprogramming and dystrophin gene addition in mdx mouse induced pluripotent stem cells. PLoS One 9(4): e96279.
  4. Geisinger, J. M. and Calos, M. P. (2012). Site-specifc recombination using phiC31 integrase. In: Renault, S. and Duchateau, P. (Eds). Site-directed insertion of transgenes. Springer, 23: 211-39.
  5. Buchner, D. A., Geisinger, J. M., Glazebrook, P. A., Morgan, M. G., Spiezio, S. H., Kaiyala, K. J., Schwartz, M. W., Sakurai, T., Furley, A. J., Kunze, D. L., Croniger, C. M. and Nadeau, J. H. (2012). The juxtaparanodal proteins CNTNAP2 and TAG1 regulate diet-induced obesity. Mamm Genome 23(7-8): 431-442.
  6. Karow, M., Chavez, C. L., Farruggio, A. P., Geisinger, J. M., Keravala, A., Jung, W. E., Lan, F., Wu, J. C., Chen-Tsai, Y. and Calos, M. P. (2011). Site-specific recombinase strategy to create induced pluripotent stem cells efficiently with plasmid DNA. Stem Cells 29(11): 1696-1704.
  7. Yazbek, S. N., Buchner, D. A., Geisinger, J. M., Burrage, L. C., Spiezio, S. H., Zentner, G. E., Hsieh, C. W., Scacheri, P. C., Croniger, C. M. and Nadeau, J. H. (2011). Deep congenic analysis identifies many strong, context-dependent QTLs, one of which, Slc35b4, regulates obesity and glucose homeostasis. Genome Res 21(7): 1065-1073.
  8. Daulny, A., Geng, F., Muratani, M., Geisinger, J. M., Salghetti, S. E. and Tansey, W. P. (2008). Modulation of RNA polymerase II subunit composition by ubiquitylation. Proc Natl Acad Sci U S A 105(50): 19649-19654.
1 Protocol published
Knock-in Blunt Ligation Utilizing CRISPR/Cas9
Authors:  Jonathan M. Geisinger and Michele P. Calos, date: 03/05/2017, view: 1715, Q&A: 0
The incorporation of the CRISPR/Cas9 bacterial immune system into the genetic engineering toolbox has led to the development of several new methods for genome manipulation (Auer et al., 2014; Byrne et al., 2015). We took advantage ...