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Coauthors
Hajime Wada Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Japan
2 protocols

Shin-ya Miyagishima Department of Cell Genetics, National Institute of Genetics, Japan
2 protocols
Coreviewers
Arsalan Daudi University of California
153 protocols

Baohua Li University of California, Davis
4 protocols

Reviewer
Kumiko Okazaki
  • Assistant professor, Hiroshima University
Research focus
  • Plant science
Contributions
  • 2 Author merit
  • 1 Reviewer merit

Education

Ph.D. (Science), The University of Tokyo

2 Protocols published
Preparation of Chloroplast Lipid Membrane and Lipid-protein Interaction Assay
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 2656, Q&A: 0
Lipid-Protein interaction assay is a method to search lipids, which are bound with proteins in vitro. Since membranes that are spotted with chloroplast lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), ...
Measurement of PI4P Levels in Intact Chloroplasts Isolated from Arabidopsis thaliana
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 2420, Q&A: 0
Phosphatidylinositol 4-phosphate (PI4P), a major species of phosphoinositides, modulates many fundamental cellular processes. We have recently revealed that PI4P plays an important role in chloroplast division as a negative regulator. Despite its ...
1 Protocol reviewed
Fusarium graminearum Maize Stalk Infection Assay and Associated Microscopic Observation Protocol
Authors:  Juan He, Tinglu Yuan and Wei-Hua Tang, date: 12/05/2016, view: 2161, Q&A: 0
The ascomycete fungus Fusarium graminearum (previously also called Gibberella zeae) causes Gibberella stalk rot in maize (Zea mays) and results in lodging and serious yield reduction. To develop methods to assess ...
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