Search
Coauthors
Rohit Gupta Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, USA
1 protocol

Chantia Carroll Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, USA
1 protocol

Alaina Puleo Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, USA
1 protocol

Yael Rosenberg-Hasson Human Immune Monitoring Center, Stanford University, USA
1 protocol

Holden Maecker
  • Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, USA
Contributions
  • 23 Author merit

Education

Ph.D. in Cancer Biology, Stanford University, 1988

Current position

Director, Human Immune Monitoring Center, Stanford University, Stanford, CA (2009-present)
Associate Professor (Research), Microbiology & Immunology, Stanford University, Stanford, CA (2011-present)

Publications

Selected peer-reviewed publications

  1. Shen-Orr, S. S., Furman, D., Kidd, B. A., Hadad, F., Lovelace, P., Huang, Y. W., Rosenberg-Hasson, Y., Mackey, S., Grisar, F. A., Pickman, Y., Maecker, H. T., Chien, Y. H., Dekker, C. L., Wu, J. C., Butte, A. J. and Davis, M. M. (2016). Defective Signaling in the JAK-STAT Pathway Tracks with Chronic Inflammation and Cardiovascular Risk in Aging Humans. Cell Syst 3(4): 374-384 e374.
  2. Zaunders, J., Jing, J., Leipold, M., Maecker, H., Kelleher, A. D. and Koch, I. (2016). Computationally efficient multidimensional analysis of complex flow cytometry data using second order polynomial histograms. Cytometry A 89(1): 44-58.
  3. Mukai, K., Gaudenzio, N., Gupta, S., Vivanco, N., Bendall, S. C., Maecker, H. T., Chinthrajah, R. S., Tsai, M., Nadeau, K. C. and Galli, S. J. (2016). Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis. J Allergy Clin Immunol. [Epub ahead of print]
  4. Japp, A. S., Hoffmann, K., Schlickeiser, S., Glauben, R., Nikolaou, C., Maecker, H. T., Braun, J., Matzmohr, N., Sawitzki, B., Siegmund, B., Radbruch, A., Volk, H. D., Frentsch, M., Kunkel, D. and Thiel, A. (2016). Wild immunology assessed by multidimensional mass cytometry. Cytometry A. [Epub ahead of print]
  5. Yabu, J. M., Siebert, J. C. and Maecker, H. T. (2016). Immune Profiles to Predict Response to Desensitization Therapy in Highly HLA-Sensitized Kidney Transplant Candidates. PLoS One 11(4): e0153355.
  6. Ryan, J. F., Hovde, R., Glanville, J., Lyu, S. C., Ji, X., Gupta, S., Tibshirani, R. J., Jay, D. C., Boyd, S. D., Chinthrajah, R. S., Davis, M. M., Galli, S. J., Maecker, H. T. and Nadeau, K. C. (2016). Successful immunotherapy induces previously unidentified allergen-specific CD4+ T-cell subsets. Proc Natl Acad Sci U S A 113(9): E1286-1295.
  7. Finak, G., Langweiler, M., Jaimes, M., Malek, M., Taghiyar, J., Korin, Y., Raddassi, K., Devine, L., Obermoser, G., Pekalski, M. L., Pontikos, N., Diaz, A., Heck, S., Villanova, F., Terrazzini, N., Kern, F., Qian, Y., Stanton, R., Wang, K., Brandes, A., Ramey, J., Aghaeepour, N., Mosmann, T., Scheuermann, R. H., Reed, E., Palucka, K., Pascual, V., Blomberg, B. B., Nestle, F., Nussenblatt, R. B., Brinkman, R. R., Gottardo, R., Maecker, H. and McCoy, J. P. (2016). Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium. Sci Rep 6: 20686.
  8. Hiniker, S. M., Reddy, S. A., Maecker, H. T., Subrahmanyam, P. B., Rosenberg-Hasson, Y., Swetter, S. M., Saha, S., Shura, L. and Knox, S. J. (2016). A Prospective Clinical Trial Combining Radiation Therapy With Systemic Immunotherapy in Metastatic Melanoma. Int J Radiat Oncol Biol Phys 96(3): 578-588.
  9. Kojima, N., Bristow, C. C., Maecker, H., Rosenberg-Hasson, Y., Leon, S. R., Vargas, S. K., Konda, K. A., Caceres, C. F. and Klausner, J. D. (2016). Similarities in the Markers of Inflammation Between Men With Syphilis and Women With Increased Risk of HIV Acquisition. Clin Infect Dis 62(2): 265-266.
  10. Kuznetsova, T., Haddad, F., Knez, J., Rosenberg-Hasson, Y., Sung, J., Cauwenberghs, N., Thijs, L., Karakikes, I., Maecker, H., Mahaffey, K. W., Wu, J. C. and Staessen, J. A. (2015). Cytokines profile in hypertensive patients with left ventricular remodeling and dysfunction. J Am Soc Hypertens 9(12): 975-984 e973.
  11. Mei, H. E., Leipold, M. D. and Maecker, H. T. (2016). Platinum-conjugated antibodies for application in mass cytometry. Cytometry A 89(3): 292-300.
  12. Yuan, J., Hegde, P. S., Clynes, R., Foukas, P. G., Harari, A., Kleen, T. O., Kvistborg, P., Maccalli, C., Maecker, H. T., Page, D. B., Robins, H., Song, W., Stack, E. C., Wang, E., Whiteside, T. L., Zhao, Y., Zwierzina, H., Butterfield, L. H. and Fox, B. A. (2016). Novel technologies and emerging biomarkers for personalized cancer immunotherapy. J Immunother Cancer 4: 3.
  13. Maecker, H. T. (2016). New Cellular Assays.  In: Nathanson, N. (Ed.). Viral Pathogenesis, Elsevier Ltd, pp. 315-317.
  14. Whiting, C. C., Siebert, J., Newman, A. M., Du, H. W., Alizadeh, A. A., Goronzy, J., Weyand, C. M., Krishnan, E., Fathman, C. G. and Maecker, H. T. (2015). Large-Scale and Comprehensive Immune Profiling and Functional Analysis of Normal Human Aging. PLoS One 10(7): e0133627.
  15. Haddad, F., Sever, M., Poglajen, G., Lezaic, L., Yang, P., Maecker, H., Davis, M., Kuznetsova, T., Wu, J. C. and Vrtovec, B. (2015). Immunologic Network and Response to Intramyocardial CD34+ Stem Cell Therapy in Patients With Dilated Cardiomyopathy. J Card Fail 21(7): 572-582.
  16. Furman, D., Jojic, V., Sharma, S., Shen-Orr, S. S., Angel, C. J., Onengut-Gumuscu, S., Kidd, B. A., Maecker, H. T., Concannon, P., Dekker, C. L., Thomas, P. G. and Davis, M. M. (2015). Cytomegalovirus infection enhances the immune response to influenza. Sci Transl Med 7(281): 281ra243.
  17. Mei, H. E., Leipold, M. D., Schulz, A. R., Chester, C. and Maecker, H. T. (2015). Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol 194(4): 2022-2031.
  18. Brodin, P., Jojic, V., Gao, T., Bhattacharya, S., Angel, C. J., Furman, D., Shen-Orr, S., Dekker, C. L., Swan, G. E., Butte, A. J., Maecker, H. T. and Davis, M. M. (2015). Variation in the human immune system is largely driven by non-heritable influences. Cell 160(1-2): 37-47.
  19. Courtot, M., Meskas, J., Diehl, A. D., Droumeva, R., Gottardo, R., Jalali, A., Taghiyar, M. J., Maecker, H. T., McCoy, J. P., Ruttenberg, A., Scheuermann, R. H. and Brinkman, R. R. (2015). flowCL: ontology-based cell population labelling in flow cytometry. Bioinformatics 31(8): 1337-1339.
  20. Kvistborg, P., Gouttefangeas, C., Aghaeepour, N., Cazaly, A., Chattopadhyay, P. K., Chan, C., Eckl, J., Finak, G., Hadrup, S. R., Maecker, H. T., Maurer, D., Mosmann, T., Qiu, P., Scheuermann, R. H., Welters, M. J., Ferrari, G., Brinkman, R. R. and Britten, C. M. (2015). Thinking outside the gate: single-cell assessments in multiple dimensions. Immunity 42(4): 591-592.
  21. Hiniker, S. M., Maecker, H. T. and Knox, S. J. (2015). Predictors of clinical response to immunotherapy with or without radiotherapy. J Radiat Oncol 4: 339-345.
  22. Maecker, H. T. and Harari, A. (2015). Immune monitoring technology primer: flow and mass cytometry. J Immunother Cancer 3: 44.
  23. Chester, C. and Maecker, H. T. (2015). Algorithmic Tools for Mining High-Dimensional Cytometry Data. J Immunol 195(3): 773-779.
  24. Leipold, M. D., Newell, E. W. and Maecker, H. T. (2015). Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry. Methods Mol Biol 1343: 81-95.
  25. Nair, N., Mei, H. E., Chen, S. Y., Hale, M., Nolan, G. P., Maecker, H. T., Genovese, M., Fathman, C. G. and Whiting, C. C. (2015). Mass cytometry as a platform for the discovery of cellular biomarkers to guide effective rheumatic disease therapy. Arthritis Res Ther 17: 127.
  26. Mannick, J. B., Del Giudice, G., Lattanzi, M., Valiante, N. M., Praestgaard, J., Huang, B., Lonetto, M. A., Maecker, H. T., Kovarik, J., Carson, S., Glass, D. J. and Klickstein, L. B. (2014). mTOR inhibition improves immune function in the elderly. Sci Transl Med 6(268): 268ra179.
  27. Nadeau, K. C., Li, Z., Farzan, S., Koestler, D., Robbins, D., Fei, D. L., Malipatlolla, M., Maecker, H., Enelow, R., Korrick, S. and Karagas, M. R. (2014). In utero arsenic exposure and fetal immune repertoire in a US pregnancy cohort. Clin Immunol 155(2): 188-197.
  28. DiCarlo, J., Agarwal-Hashmi, R., Shah, A., Kim, P., Craveiro, L., Killen, R., Rosenberg-Hasson, Y. and Maecker, H. (2014). Cytokine and chemokine patterns across 100 days after hematopoietic stem cell transplantation in children. Biol Blood Marrow Transplant 20(3): 361-369.
  29. Rosenberg-Hasson, Y., Hansmann, L., Liedtke, M., Herschmann, I. and Maecker, H. T. (2014). Effects of serum and plasma matrices on multiplex immunoassays. Immunol Res 58(2-3): 224-233.
  30. Chang, S., Kohrt, H. and Maecker, H. T. (2014). Monitoring the immune competence of cancer patients to predict outcome. Cancer Immunol Immunother 63(7): 713-719.
  31. Leipold, M. and Maecker, H. T. (2013). Multiparameter phenotyping of human PBMCs using mass cytometry. In: Shaw, A. (ed). Immunosenescence.
  32. Furman, D., Jojic, V., Kidd, B., Shen-Orr, S., Price, J., Jarrell, J., Tse, T., Huang, H., Lund, P., Maecker, H. T., Utz, P. J., Dekker, C. L., Koller, D. and Davis, M. M. (2013). Apoptosis and other immune biomarkers predict influenza vaccine responsiveness. Mol Syst Biol 9: 659.
  33. Horowitz, A., Strauss-Albee, D. M., Leipold, M., Kubo, J., Nemat-Gorgani, N., Dogan, O. C., Dekker, C. L., Mackey, S., Maecker, H., Swan, G. E., Davis, M. M., Norman, P. J., Guethlein, L. A., Desai, M., Parham, P. and Blish, C. A. (2013). Genetic and environmental determinants of human NK cell diversity revealed by mass cytometry. Sci Transl Med 5(208): 208ra145.
  34. Britten, C. M., Janetzki, S., Butterfield, L. H., Ferrari, G., Gouttefangeas, C., Huber, C., Kalos, M., Levitsky, H. I., Maecker, H. T., Melief, C. J., O'Donnell-Tormey, J., Odunsi, K., Old, L. J., Ottenhoff, T. H., Ottensmeier, C., Pawelec, G., Roederer, M., Roep, B. O., Romero, P., van der Burg, S. H., Walter, S., Hoos, A. and Davis, M. M. (2012). T cell assays and MIATA: the essential minimum for maximum impact. Immunity 37(1): 1-2.
  35. Leipold, M. D. and Maecker, H. T. (2012). Mass cytometry: protocol for daily tuning and running cell samples on a CyTOF mass cytometer. J Vis Exp(69): e4398.
  36. Maecker, H. T., McCoy, J. P. and Nussenblatt, R. (2012). Standardizing immunophenotyping for the Human Immunology Project. Nat Rev Immunol 12(3): 191-200.
  37. Siebert, J. C., Munsil, W., Rosenberg-Hasson, Y., Davis, M. M. and Maecker, H. T. (2012). The Stanford Data Miner: a novel approach for integrating and exploring heterogeneous immunological data. J Transl Med 10: 62.
  38. Streitz, M., Fuhrmann, S., Thomas, D., Cheek, E., Nomura, L., Maecker, H., Martus, P., Aghaeepour, N., Brinkman, R. R., Volk, H. D. and Kern, F. (2012). The phenotypic distribution and functional profile of tuberculin-specific CD4 T-cells characterizes different stages of TB infection. Cytometry B Clin Cytom 82(6): 360-368.
  39. Streitz, M., Fuhrmann, S., Powell, F., Quassem, A., Nomura, L., Maecker, H., Martus, P., Volk, H. D. and Kern, F. (2011). Tuberculin-specific T cells are reduced in active pulmonary tuberculosis compared to LTBI or status post BCG vaccination. J Infect Dis 203(3): 378-382.
  40. Jaimes, M. C., Maecker, H. T., Yan, M., Maino, V. C., Hanley, M. B., Greer, A., Darden, J. M. and D'Souza, M. P. (2011). Quality assurance of intracellular cytokine staining assays: analysis of multiple rounds of proficiency testing. J Immunol Methods 363(2): 143-157.
  41. Maecker, H. T., McCoy, J. P., Jr., Consortium, F. H. I., Amos, M., Elliott, J., Gaigalas, A., Wang, L., Aranda, R., Banchereau, J., Boshoff, C., Braun, J., Korin, Y., Reed, E., Cho, J., Hafler, D., Davis, M., Fathman, C. G., Robinson, W., Denny, T., Weinhold, K., Desai, B., Diamond, B., Gregersen, P., Di Meglio, P., Nestle, F. O., Peakman, M., Villanova, F., Ferbas, J., Field, E., Kantor, A., Kawabata, T., Komocsar, W., Lotze, M., Nepom, J., Ochs, H., O'Lone, R., Phippard, D., Plevy, S., Rich, S., Roederer, M., Rotrosen, D. and Yeh, J. H. (2010). A model for harmonizing flow cytometry in clinical trials. Nat Immunol 11(11): 975-978.
  42. Janetzki, S., Britten, C. M., Kalos, M., Levitsky, H. I., Maecker, H. T., Melief, C. J., Old, L. J., Romero, P., Hoos, A. and Davis, M. M. (2009). "MIATA"-minimal information about T cell assays. Immunity 31(4): 527-528.
7 Protocols published
Isolation of PBMCs Using Vacutainer® Cellular Preparation Tubes (CPTTM)
Authors:  Alaina Puleo, Chantia Carroll, Holden Maecker and Rohit Gupta, date: 01/20/2017, view: 3715, Q&A: 0
Peripheral blood mononuclear cell (PBMC) isolation is commonly done via density gradient centrifugation over Ficoll-Hypaque, a labor-intensive procedure that requires skilled technicians and can contribute to sample variability. Cellular Preparation ...
Cryopreservation of Human Serum
Authors:  Yael Rosenberg-Hasson and Holden Maecker, date: 11/05/2015, view: 1969, Q&A: 0
This protocol describes how to collect and aliquot human serum and store at -80 °C for future cytokine/chemokine/protein profile analysis.
Please use personal protective equipment (PPE) as required when handling samples with potential ...