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Coauthors
Ming Chang Center for Plant Biology, School of Life Sciences, Tsinghua University, China
1 protocol

Yuxiang Jiang Center for Plant Biology, School of Life Sciences, Tsinghua University, China
1 protocol

Yurong Xie Chinese Academy of Agricultural Sciences
1 protocol

Shanjin Huang
  • Center for Plant Biology, School of Life Sciences, Tsinghua University, China
  • 3 Author merit

Education

Ph.D in Plant Sciences, College of Biological Sciences, China Agricultural University, 2001

Current position

Principal Investigator at Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China

Publications

  1. Zheng, Y., Xie, Y., Jiang, Y., Qu, X. and Huang, S. (2013). Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth. Plant Cell 25(9): 3405-3423.
  2. Qu, X., Zhang, H., Xie, Y., Wang, J., Chen, N. and Huang, S. (2013). Arabidopsis villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars. Plant Cell 25(5): 1803-1817.
  3. Shi, M., Xie, Y., Zheng, Y., Wang, J., Su, Y., Yang, Q. and Huang, S. (2013). Oryza sativa actin-interacting protein 1 is required for rice growth by promoting actin turnover. Plant J 73(5): 747-760.
  4. Zheng, Y., Xin, H., Lin, J., Liu, C. M. and Huang, S. (2012). An Arabidopsis class II formin, AtFH19, nucleates actin assembly, binds to the barbed end of actin filaments, and antagonizes the effect of AtFH1 on actin dynamics. J Integr Plant Biol 54(10): 800-813.
  5. Bao, C., Wang, J., Zhang, R., Zhang, B., Zhang, H., Zhou, Y. and Huang, S. (2012). Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments. Plant J 71(6): 962-975.
  6. Zhao, Y., Zhao, S., Mao, T., Qu, X., Cao, W., Zhang, L., Zhang, W., He, L., Li, S., Ren, S., Zhao, J., Zhu, G., Huang, S., Ye, K., Yuan, M. and Guo, Y. (2011). The plant-specific actin binding protein SCAB1 stabilizes actin filaments and regulates stomatal movement in Arabidopsis. Plant Cell 23(6): 2314-2330.
  7. Yang, W., Ren, S., Zhang, X., Gao, M., Ye, S., Qi, Y., Zheng, Y., Wang, J., Zeng, L., Li, Q., Huang, S. and He, Z. (2011). BENT UPPERMOST INTERNODE1 encodes the class II formin FH5 crucial for actin organization and rice development. Plant Cell 23(2): 661-680.
  8. Wu, Y., Yan, J., Zhang, R., Qu, X., Ren, S., Chen, N. and Huang, S. (2010). Arabidopsis FIMBRIN5, an actin bundling factor, is required for pollen germination and pollen tube growth. Plant Cell 22(11): 3745-3763.
  9. Zhang, H., Qu, X., Bao, C., Khurana, P., Wang, Q., Xie, Y., Zheng, Y., Chen, N., Blanchoin, L., Staiger, C. J. and Huang, S. (2010). Arabidopsis VILLIN5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth. Plant Cell 22(8): 2749-2767.
  10. Zhu, Y., Wang, Y., Li, R., Song, X., Wang, Q., Huang, S., Jin, J. B., Liu, C. M. and Lin, J. (2010). Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis. Plant J 61(2): 223-233.
  11. Wang, J., Wang, H. Y., Zhao, P. M., Han, L. B., Jiao, G. L., Zheng, Y. Y., Huang, S. J. and Xia, G. X. (2010). Overexpression of a profilin (GhPFN2) promotes the progression of developmental phases in cotton fibers. Plant Cell Physiol 51(8): 1276-1290.
  12. Chen, N., Qu, X., Wu, Y. and Huang, S. (2009). Regulation of actin dynamics in pollen tubes: control of actin polymer level. J Integr Plant Biol 51(8): 740-750.
  13. Ye, J., Zheng, Y., Yan, A., Chen, N., Wang, Z., Huang, S. and Yang, Z. (2009). Arabidopsis formin3 directs the formation of actin cables and polarized growth in pollen tubes. Plant Cell 21(12): 3868-3884.
  14. Yang, G., Gao, P., Zhang, H., Huang, S. and Zheng, Z. L. (2007). A mutation in MRH2 kinesin enhances the root hair tip growth defect caused by constitutively activated ROP2 small GTPase in Arabidopsis. PLoS ONE 2(10): e1074.
3 Protocols published
Rapid Isolation of Total Protein from Arabidopsis Pollen
Authors:  Ming Chang and Shanjin Huang, date: 04/20/2017, view: 1220, Q&A: 0
Arabidopsis pollen is an excellent system for answering important biological questions about the establishment and maintenance of cellular polarity and polar cell growth, because these processes are amenable to the genetic and genomic ...
Direct Visualization and Quantification of the Actin Nucleation and Elongation Events in vitro by TIRF Microscopy
Authors:  Yuxiang Jiang and Shanjin Huang, date: 03/05/2017, view: 1473, Q&A: 0
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing the dynamics of actin filaments at single-filament resolution in vitro. Thanks to the development of various fluorescent probes, we can easily ...