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Coauthors
Scott Lowe Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, USA
2 protocols

Lukas Dow Department of Medicine, Weill Cornell Medical College, USA
2 protocols

Sarah Ackerman Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, USA
1 protocol
Coreviewers
Andrea Puhar MIMS - Umea University
18 protocols

HongLok Lung University of Hong Kong
56 protocols

Vikash Verma University of Massachusetts
4 protocols

Reviewer
Kevin Patrick O’Rourke
  • Graduate, Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program
Research focus
  • Cancer biology
  • Functional genomics
    Mouse models
  • 2 Author merit

Education

BA, Middlebury College

Lab information

Scott Lowe

2 Protocols published
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
Authors:  Kevin P. O’Rourke, Sarah Ackerman, Lukas E Dow and Scott W Lowe, date: 02/20/2016, view: 7325, Q&A: 3
In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and ...
Immunofluorescent Staining of Mouse Intestinal Stem Cells
Authors:  Kevin P. O’Rourke, Lukas E Dow and Scott W Lowe, date: 02/20/2016, view: 8188, Q&A: 0
Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including ...
2 Protocols reviewed
Teratoma Formation Assay for Assessing Pluripotency and Tumorigenicity of Pluripotent Stem Cells
Authors:  Shingo Miyawaki, Yohei Okada, Hideyuki Okano and Kyoko Miura, date: 08/20/2017, view: 1142, Q&A: 0
Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma ...
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Generation of Tumour-stroma Minispheroids for Drug Efficacy Testing
Authors:  Mark Watters and Eva Szegezdi, date: 01/05/2017, view: 1958, Q&A: 0
The three-dimensional organisation of cells in a tissue and their interaction with adjacent cells and extracellular matrix is a key determinant of cellular responses, including how tumour cells respond to stress conditions or therapeutic drugs ...
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