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Coauthors
Robin Farhaeus Cibles Therapeutiques, INSERM Unite 940, Universite Paris 7, France
2 protocols
Coreviewers
Antoine de Morree Stanford university
27 protocols

HongLok Lung University of Hong Kong
56 protocols

Isabel Silva University of São Paulo State - UNESP
5 protocols

Lee-Hwa Tai Universite de Sherbrooke
20 protocols

Reviewer
Vanesa Olivares-Illana
  • Research scientist, Instituto de Física, Universidad Autónoma de San Luis Potosí
Research focus
  • Cancer biology
  • Biochemistry, cancer, interactions, retinoblastoma, p53
  • 2 Author merit

Education

PhD, UNAM, 2003

Lab information

Vanesa Olivares Illana Labs

2 Protocols published
Purification of His-ubiquitin Proteins from Mammalian Cells
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 10332, Q&A: 6
This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just ...
Protein-RNA ELISA Assay
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 6084, Q&A: 0
Protein-RNA ELISA assay is an effective and quantitative method to study protein-RNA interactions in vitro. In this protocol we used recombinant 6x HIS tagged protein, but it works as well for non tagged proteins.
13 Protocols reviewed
Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis
Authors:  Feng Du, Xiaodi Zhao and Daiming Fan, date: 06/20/2017, view: 1463, Q&A: 0
Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified first, followed by an upper layer containing a ...
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Virtual Screening of Transmembrane Serine Protease Inhibitors
Authors:  Antti Poso, Topi Tervonen and Juha Klefström, date: 04/20/2017, view: 1346, Q&A: 0
The human family of type II transmembrane serine proteases includes 17 members. The defining features of these proteases are an N-terminal transmembrane domain and a C-terminal serine protease of the chymotrypsin (S1) fold, separated from each other ...
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