Search
Coreviewers
Fanglian He Department of Biology, University of Pennsylvania, USA
205 protocols

Hui Zhu Department of Medicine, Stanford University, USA
17 protocols

Omar Akil Department of Otolaryngology-Head & Neck Surgery, University of California San Francisco, USA
7 protocols

Ralph Boettcher Molecular Medicine Division, Max Planck Institute for Biochemistry, Germany
18 protocols

Lin Fang
  • Department of Pediatrics, School of Medicine, Stanford University, USA
Research focus
  • Cell biology
Contributions
  • 4 Author merit
  • 18 Reviewer merit
  • 77 Editor merit

Education

PhD, University of Oregon, 2016

4 Protocols published
Antibody Purification from Western Blotting
Author:  Lin Fang, date: 03/20/2012, view: 9957, Q&A: 2
This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in ...
Expression and Purification of GST-tagged Proteins from E. coli
Author:  Lin Fang, date: 09/20/2011, view: 11784, Q&A: 1
This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
18 Protocols reviewed
Actin Retrograde Flow in Permeabilized Cells: Myosin-II Driven Centripetal Movement of Transverse Arcs
Authors:  Yee Han Tee and Alexander D. Bershadsky, date: 03/05/2016, view: 1959, Q&A: 0
Numerous biological functions such as cytokinesis, changes in cell shape and cell migration require actomyosin-driven cellular contractility. However, the detailed mechanism of how contractile forces drive cellular processes are difficult to ...
More >
Three Dimensional Spheroid Co-culture Invasion Assay
Authors:  Eldo T Verghese and Thomas A Hughes, date: 01/05/2015, view: 4487, Q&A: 0
The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells ...
More >
77 Protocols edited
Flow Cytometric Detection of Mitochondrial Membrane Potential
Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function and an indicator of cell health. Depletion of Δψm suggests the loss of mitochondrial membrane integrity reflecting the initiation of the proapoptotic signal. ...
More >
Lung Clearance Assay
Authors:  Pinchas Tsukerman and Ofer Mandelboim, date: 03/05/2013, view: 4233, Q&A: 0
Lung clearance assay tests the ability of innate immune cells (mainly NK cells) to eradicate in vivo cells injected via the tail vein of the mice. This assay helps to elucidate the role played by NK cells and their receptors (if the mice ...
More >