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Coauthors
Gyorgy Szabadkai Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
1 protocol

Michael Duchen Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
1 protocol

Tunde Berecz Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
1 protocol

Tom S. Blacker
  • Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
  • 1 Author merit

Education

Ph.D in Biophysics, University College London, 2013

Publications

  1. Blacker, T. S., Duchen, M. R. (2016). Investigating mitochondrial redox state using NADH and NADPH autofluorescence. Free Radical Biology and Medicine 100: 53-65.
  2. Tosatto, A., Sommaggio, R., Kummerow, C., Bentham, R. B., Blacker, T. S., Berecz, T., Duchen, M. R., Rosato, A., Bogeski, I., Szabadkai, G., Rizzuto, R. and Mammucari, C. (2016). The mitochondrial calcium uniporter regulates breast cancer progression via HIF-1alpha. EMBO Mol Med 8(5): 569-585.
  3. Blacker, T. S., Duchen, M. R. and Bain, A. J. (2016). Heterogeneity and restricted state selection in FRET with fluorescent proteins. SPIE BiOS 971401-971401-16.
  4. Nickel, A. G., von Hardenberg, A., Hohl, M., Loffler, J. R., Kohlhaas, M., Becker, J., Reil, J. C., Kazakov, A., Bonnekoh, J., Stadelmaier, M., Puhl, S. L., Wagner, M., Bogeski, I., Cortassa, S., Kappl, R., Pasieka, B., Lafontaine, M., Lancaster, C. R., Blacker, T. S., Hall, A. R., Duchen, M. R., Kastner, L., Lipp, P., Zeller, T., Muller, C., Knopp, A., Laufs, U., Bohm, M., Hoth, M. and Maack, C. (2015). Reversal of Mitochondrial Transhydrogenase Causes Oxidative Stress in Heart Failure. Cell Metab 22(3): 472-484.
  5. Blacker, T. S., Mann, Z. F., Gale, J. E., Ziegler, M., Bain, A. J., Szabadkai, G. and Duchen, M. R. (2014). Separating NADH and NADPH fluorescence in live cells and tissues using FLIM. Nat Commun 5: 3936.
  6. Blacker, T. S., Marsh, R. J., Duchen, M. R. and Bain, A. J. (2013). Activated barrier crossing dynamics in the non-radiative decay of NADH and NADPH. Chemical Physics. 422: 184-194.
  7. Osellame, L. D., Blacker, T. S. and Duchen, M. R. (2012). Cellular and molecular mechanisms of mitochondrial function. Best Practice & Research Clinical Endocrinology & Metabolism 26(6): 711-723.
  8. Nicolaou, N., Marsh, R. J., Blacker, T. S., Armoogum, D. A. and Bain, A. J. (2009). Time-resolved fluorescence and FCS studies of dye-doped DNA. Proc SPIE  7393.
1 Protocol published
Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime
Authors:  Thomas S. Blacker, Tunde Berecz, Michael R. Duchen and Gyorgy Szabadkai, date: 01/20/2017, view: 2305, Q&A: 0
NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role in cellular antioxidant defence. In live cells and tissues, the intensity of their ...