Search
Coauthors
Andrew Norton Durham University Business School, Durham University, UK
1 protocol

Yvette Coulson-Thomas Department of Biochemistry, Universidade Federal de São Paulo, Brazil
2 protocols

Tarsis Gesteira Ferreira University of Cincinnati
1 protocol
Coreviewers
Alka Mehra Institute of Genomics and Integrative Biology
14 protocols

Federica Pisano Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo
4 protocols

Guillermo Gomez University of South Australia
5 protocols

Kristina Aguilera University of California, Los Angeles
2 protocols

Editor
Vivien J. Coulson-Thomas
  • Assistant professor, University of Houston
Research focus
  • Stem cell
  • 4 Author merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=coulson-+thomas+vj

4 Protocols published
Isolation and Analysis of Proteoglycans and Glycosaminoglycans from Archaeological Bones and Teeth
Authors:  Yvette May Coulson-Thomas, Vivien Jane Coulson-Thomas and Andrew L. Norton, date: 04/05/2017, view: 1253, Q&A: 0
We have developed methods for isolating proteoglycans and glycosaminoglycans from archaeological bones and teeth. These methods have been previously published (Coulson-Thomas et al., 2015) and are described here in more detail. In the case ...
3D Stroma Invasion Assay
Authors:  Yvette May Coulson-Thomas and Vivien Jane Coulson-Thomas, date: 03/20/2017, view: 1307, Q&A: 0
We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of ...
5 Protocols reviewed
In vitro Chondrogenic Hypertrophy Induction of Mesenchymal Stem Cells
Authors:  Sang Young Jeong, Miyoung Lee, Soo Jin Choi, Wonil Oh and Hong Bae Jeon, date: 12/05/2016, view: 2491, Q&A: 0
To investigate underlying mechanism of chondrogenic hypertrophy, we need proper in vitro hypertrophic model of mesenchymal stem cells (MSCs). This protocol describes our defined method for induction of in vitro chondrogenic ...
More >
Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures
Authors:  Xiurong Zhao, Liyan Zhang, Shun-Ming Ting and Jaroslaw Aronowski, date: 04/20/2016, view: 2700, Q&A: 0
Clearance of dead brain tissue including the dead neurons through phagocytosis is an endogenous function of microglia in the brain, which is critical for inflammation resolution after ischemic stroke or head trauma. By regulating the function or ...
More >
5 Protocols edited
Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging
Fluorescence live-cell imaging by single molecule localization microscopy (SMLM) or fluorescence lifetime imaging microscopy (FLIM) in principle allows for the spatio-temporal observation of molecular patterns in individual, living cells. However, ...
More >
Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition
Authors:  Susan M. Carnachan and Simon F.R. Hinkley, date: 04/05/2017, view: 1289, Q&A: 0
Heparan sulfate (HS) is purified from complex matrices and often not fully characterised to validate its assignment. The characterisation of heparins and heparan sulfates through enzymatic depolymerisation and subsequent strong anion-exchange high ...
More >