Pedro Rodruiguez Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, Spain
1 protocol

Roberto Gaxiola School of Life Sciences, Arizona State University, USA
1 protocol

Kamesh Regmi School of Life Sciences, Arizona State University, USA
1 protocol
HongLok Lung Department of Clinical Oncology and Center for Cancer Research, The University of Hong Kong, Hong Kong SAR
56 protocols

Tie Liu Carnegie Institution for Science, Stanford University
147 protocols

Gaston A. Pizzio
  • Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG; consortium CSIC-IRTA-UAB-UB), Spain
Research focus
  • Plant science
  • 2 Author merit
  • 2 Reviewer merit


Ph.D in Basic and Applied Science, National University of Quilmes (UNQ), Argentina, 2009

Current position

Post-doctoral position, Department of Plant Development and Signal. CENTRE FOR RESEARCH IN AGRICULTURAL GENOMICS – CRAG (Csic-UAB-UB-IRTA. Bellaterra (Cerdanyola del Vallés), Barcelona, Spain


  1. Pizzio, G. A., Regmi, K. and Gaxiola, R. (2015). Rhizosphere acidification assay. Bio-protocol 5(23): e1676. 
  2. Khadilkar, A. S., Yadav, U. P., Salazar, C., Shulaev, V., Paez-Valencia, J., Pizzio, G. A., Gaxiola, R. A. and Ayre, B. G. (2015). Constitutive and companion cell-specific overexpression of AVP1, encoding a proton-pumping pyrophosphatase, enhances biomass accumulation, phloem loading and long-distance transport. Plant physiology: pp. 01409.02015.
  3. Gaxiola, R. A., Regmi, K., Paez-Valencia, J., Pizzio, G. and Zhang, S. (2015). Plant H+-PPases: Reversible enzymes with contrasting functions dependent on membrane environment. Molecular Plant.[Epub ahead of print]
  4. Pizzio, G. A., Paez-Valencia, J., Khadilkar, A. S., Regmi, K., Patron-Soberano, A., Zhang, S., Sanchez-Lares, J., Furstenau, T., Li, J., Sanchez-Gomez, C., Valencia-Mayoral, P., Yadav, U. P., Ayre, B. G. and Gaxiola, R. A. (2015). Arabidopsis type I proton-pumping pyrophosphatase expresses strongly in phloem, where it is required for pyrophosphate metabolism and photosynthate partitioning. Plant Physiol 167(4): 1541-1553.
  5. Pizzio, G. A., Rodriguez, L., Antoni, R., Gonzalez-Guzman, M., Yunta, C., Merilo, E., Kollist, H., Albert, A. and Rodriguez, P. L. (2013). The PYL4 A194T mutant uncovers a key role of PYR1-LIKE4/PROTEIN PHOSPHATASE 2CA interaction for abscisic acid signaling and plant drought resistance. Plant Physiol 163(1): 441-455.
  6. Antoni, R., Gonzalez-Guzman, M., Rodriguez, L., Peirats-Llobet, M., Pizzio, G. A., Fernandez, M. A., De Winne, N., De Jaeger, G., Dietrich, D., Bennett, M. J. and Rodriguez, P. L. (2013). PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root. Plant Physiol 161(2): 931-941.
  7. Gonzalez-Guzman, M.*, Pizzio, G. A.*, Antoni, R., Vera-Sirera, F., Merilo, E., Bassel, G. W., Fernandez, M. A., Holdsworth, M. J., Perez-Amador, M. A., Kollist, H. and Rodriguez, P. L. (2012). Arabidopsis PYR/PYL/RCAR receptors play a major role in quantitative regulation of stomatal aperture and transcriptional response to abscisic acid. Plant Cell 24(6): 2483-2496. (*Both authors contributed equally to this study)
  8. Antoni, R., Gonzalez-Guzman, M., Rodriguez, L., Rodrigues, A., Pizzio, G. A. and Rodriguez, P. L. (2012). Selective inhibition of clade A phosphatases type 2C by PYR/PYL/RCAR abscisic acid receptors. Plant Physiol 158(2): 970-980.
  9. Antoni, R., Rodriguez, L., Gonzalez-Guzman, M., Pizzio, G. A. and Rodriguez, P. L. (2011). News on ABA transport, protein degradation, and ABFs/WRKYs in ABA signaling. Curr Opin Plant Biol 14(5): 547-553.
  10. Pizzio, G. A. and Golombek, D. A. (2008). Photic regulation of map kinase phosphatases MKP1/2 and MKP3 in the hamster suprachiasmatic nuclei. J Mol Neurosci 34(2): 187-192.
  11. Hainich, E. C.*, Pizzio, G. A.* and Golombek, D. A. (2006). Constitutive activation of the ERK-MAPK pathway in the suprachiasmatic nuclei inhibits circadian resetting. FEBS Lett 580(28-29): 6665-6668. (*both authors contributed equally to this study)
  12. Golombek, D. A. and Pizzio, G. A. (2007). Chronobiological Aspects of Melatonin Activity. Melatonin: From Molecules to Therapy: 177.
  13. Pizzio, G. A., Hainich, E. C., Plano, S. A., Ralph, M. R. and Golombek, D. A. (2005). Nerve growth factor-induced circadian phase shifts and MAP kinase activation in the hamster suprachiasmatic nuclei. Eur J Neurosci 22(3): 665-671.
  14. Pizzio, G. A., Hainich, E. C., Ferreyra, G. A., Coso, O. A. and Golombek, D. A. (2003). Circadian and photic regulation of ERK, JNK and p38 in the hamster SCN. Neuroreport 14(11): 1417-1419.
  15. Golombek, D. A., Ferreyra, G. A., Agostino, P. V., Murad, A. D., Rubio, M. F., Pizzio, G. A., Katz, M. E., Marpegan, L. and Bekinschtein, T. A. (2003). From light to genes: moving the hands of the circadian clock. Front Biosci 8: s285-293.
2 Protocols published
In Gel Kinase Assay
Authors:  Gaston A. Pizzio and Pedro L. Rodriguez, date: 03/05/2017, view: 1575, Q&A: 0
Proper spatiotemporal regulation of protein phosphorylation in cells and tissues is required for normal development and homeostasis. We present the protocol ‘In Gel Kinase Assay’, which is useful for protein kinase activity measurements from crude ...
Rhizosphere Acidification Assay
Authors:  Gaston A. Pizzio, Kamesh Regmi and Roberto Gaxiola, date: 12/05/2015, view: 1984, Q&A: 0
Plant survival depends on the ability of root systems to establish themselves in locations where water and nutrients are available for uptake and translocation (Hawes et al., 2003). Rhizosphere influences crop productivity by mediating ...
2 Protocols reviewed
In vitro Assessment of RNA Polymerase I Activity
Author:  Marzia Govoni, date: 02/05/2017, view: 1262, Q&A: 0
In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs ...
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Resin-embedded Thin-section Immunohistochemistry Coupled with Triple Cellular Counterstaining
Authors:  William M Palmer, John W Patrick and Yong-Ling Ruan, date: 04/05/2017, view: 673, Q&A: 0
This protocol was developed to study protein localisation within the vascular bundles of developing tomato fruit however, it can be applied to any resin embedded plant tissue. The vascular bundle is comprised of many different cells that all have ...
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