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Coauthors
Rachael Giersch Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
1 protocol

Jeremy Thorner Division of Biochemistry, Biophysics and Structural Biology, Department of Molecular and Cell Biology, University of California, USA
1 protocol
Coreviewers
Ralph Boettcher Molecular Medicine Division, Max Planck Institute for Biochemistry, Germany
18 protocols

Gregory C. Finnigan
  • Biochemistry and Molecular Biophysics, Kansas State University, USA
Research focus
  • Molecular biology
Contributions
  • 2 Author merit
  • 2 Reviewer merit

Education

Ph.D. in Molecular Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR, 2011

Current position

Assistant professor, Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, USA

Publications (since 2011)

  1. Roggenkamp, E., Giersch, R. M., Wedeman, E., Eaton, M., Turnquist, E., Schrock, M. N., Alkotami, L., Jirakittisonthon, T., Schluter-Pascua, S. E., Bayne, G. H., Wasko, C., Halloran, M. and Finnigan, G. C. (2017). CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains. Front Microbiol.
  2. Roelants, F. M., Leskoske, K. L., Pedersen, R. T., Muir, A., Liu, J. M., Finnigan, G. C. and Thorner, J. (2017). TOR Complex 2-Regulated Protein Kinase Fpk1 Stimulates Endocytosis via Inhibition of Ark1/Prk1-Related Protein Kinase Akl1 in Saccharomyces cerevisiae. Mol Cell Biol 37(7).
  3. Perez, A. M., Finnigan, G. C., Roelants, F. M. and Thorner, J. (2016). Septin-Associated Protein Kinases in the Yeast Saccharomyces cerevisiae. Front Cell Dev Biol 4: 119.
  4. Finnigan, G. C., Duvalyan, A., Liao, E. N., Sargsyan, A. and Thorner, J. (2016). Detection of protein-protein interactions at the septin collar in Saccharomyces cerevisiae using a tripartite split-GFP system. Mol Biol Cell 27(17): 2708-2725.
  5. Finnigan, G. C., Sterling, S. M., Duvalyan, A., Liao, E. N., Sargsyan, A., Garcia, G., 3rd, Nogales, E. and Thorner, J. (2016). Coordinate action of distinct sequence elements localizes checkpoint kinase Hsl1 to the septin collar at the bud neck in Saccharomyces cerevisiae. Mol Biol Cell 27(14): 2213-2233.
  6. Finnigan, G. C. and Thorner, J. (2016). mCAL: A New Approach for Versatile Multiplex Action of Cas9 Using One sgRNA and Loci Flanked by a Programmed Target Sequence. G3 (Bethesda) 6(7): 2147-2156.
  7. Garcia, G., 3rd, Finnigan, G. C., Heasley, L. R., Sterling, S. M., Aggarwal, A., Pearson, C. G., Nogales, E., McMurray, M. A. and Thorner, J. (2016). Assembly, molecular organization, and membrane-binding properties of development-specific septins. J Cell Biol 212(5): 515-529.
  8. Finnigan, G. C., Booth, E. A., Duvalyan, A., Liao, E. N. and Thorner, J. (2015). The Carboxy-Terminal Tails of Septins Cdc11 and Shs1 Recruit Myosin-II Binding Factor Bni5 to the Bud Neck in Saccharomyces cerevisiae. Genetics 200(3): 843-862.
  9. Finnigan, G. C., Takagi, J., Cho, C. and Thorner, J. (2015). Comprehensive genetic analysis of paralogous terminal septin subunits Shs1 and Cdc11 in Saccharomyces cerevisiae. Genetics. (Epub ahead of print)
  10. Finnigan, G. C., Hanson-Smith, V., Stevens, T. H. and Thornton, J. W. (2012). Evolution of increased complexity in a molecular machine. Nature 481(7381): 360-364.
  11. Finnigan, G. C., Cronan, G. E., Park, H. J., Srinivasan, S., Quiocho, F. A. and Stevens, T. H. (2012). Sorting of the yeast vacuolar-type, proton-translocating ATPase enzyme complex (V-ATPase): identification of a necessary and sufficient Golgi/endosomal retention signal in Stv1p. J Biol Chem 287(23): 19487-19500.
  12. Finnigan, G. C., Ryan, M. and Stevens, T. H. (2011). A genome-wide enhancer screen implicates sphingolipid composition in vacuolar ATPase function in Saccharomyces cerevisiae. Genetics 187(3): 771-783.
  13. Finnigan, G. C., Hanson-Smith, V., Houser, B. D., Park, H. J. and Stevens, T. H. (2011). The reconstructed ancestral subunit a functions as both V-ATPase isoforms Vph1p and Stv1p in Saccharomyces cerevisiae. Mol Biol Cell 22(17): 3176-3191.
2 Protocols published
Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
Authors:  Rachael M. Giersch and Gregory C. Finnigan, date: 09/20/2017, view: 564, Q&A: 0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
Complex in vivo Ligation Using Homologous Recombination and High-efficiency Plasmid Rescue from Saccharomyces cerevisiae
Authors:  Gregory C. Finnigan and Jeremy Thorner, date: 07/05/2015, view: 2860, Q&A: 0
The protocols presented here allow for the facile generation of a wide variety of complex multipart DNA constructs (tagged gene products, gene fusions, chimeric proteins, and other variants) using homologous recombination and in vivo ...
2 Protocols reviewed
Trypsin Sensitivity Assay to Study the Folding Status of Proteins
Authors:  Satoshi Ninagawa and Kazutoshi Mori, date: 10/05/2016, view: 1619, Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing trypsin sensitivity. Unfolded/misfolded proteins are more susceptible to trypsin than folded proteins, because trypsin easily accesses and cleaves loosely folded parts of proteins. ...
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PNGase Sensitivity Assay to Study the Folding Status of Proteins
Authors:  Satoshi Ninagawa and Kazutoshi Mori, date: 10/05/2016, view: 1440, Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing peptide:N-glycanase (PNGase) sensitivity. In the cytosol, PNGase works as a deglycosylation-enzyme. N-glycans on unfolded/misfolded proteins are more susceptible to PNGase than ...
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