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Coauthors
Christopher Aiken Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, USA
1 protocol
Coreviewers
Antoine de Morree Stanford university
23 protocols

Feng Li College of horticultural and forestry sciences, Huazhong Agricultural University, Wuhan,China
12 protocols

Gal Haimovich Weizmann Institute of Science
10 protocols

Ralph Boettcher Molecular Medicine Division, Max Planck Institute for Biochemistry, Germany
18 protocols

Vaibhav B. Shah
  • Research scientist, Single Molecule Science, School of Medical Sciences, UNSW Australia
Research focus
  • Microbiology
  • virology, proteomics, molecular biology
Contributions
  • 1 Author merit
  • 8 Reviewer merit
1 Protocol published
Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells
Authors:  Vaibhav B Shah and Christopher Aiken, date: 10/20/2013, view: 4993, Q&A: 0
This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.
8 Protocols reviewed
RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells
Authors:  Michal Domanski and John LaCava, date: 04/20/2017, view: 1079, Q&A: 0
The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [Kilchert et al ...
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Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
Authors:  Lisha Shen, Zhe Liang and Hao Yu, date: 01/05/2017, view: 1778, Q&A: 0
N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m6A can be detected by several methods, such as dot blot analysis using specific m6 ...
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