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Suckjoon Jun UC San Diego, Department of Physics and Section of Molecular Biology, Division of Biology, USA, USA,
1 protocol

Xin-tian Li 1 protocol

Cindy Sou
Contributions
  • 1 Author merit
1 Protocol published
Protocol for Construction of a Tunable CRISPR Interference (tCRISPRi) Strain for Escherichia coli
Authors:  Xin-tian Li, Cindy Sou and Suckjoon Jun, date: 10/05/2017, view: 748, Q&A: 0
We present a protocol for construction of tunable CRISPR interference (tCRISPRi) strains for Escherichia coli. The tCRISPRi system alleviates most of the known problems of plasmid-based expression methods, and can be immediately used to ...