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Coauthors
Alejandro Buschiazzo Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Felipe Trajtenberg Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Ariel Mechaly Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Juan Imelio Laboratory of Molecular and Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Nicole Larrieux
  • Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
Contributions
  • 1 Author merit

Education

Clinical Biochemist, Universidad de la República, Uruguay, 2007

Current position

Technical Assistant, Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay

Publications

  1. San Martin, F., Mechaly, A. E., Larrieux, N., Wunder, E. A., Jr., Ko, A. I., Picardeau, M., Trajtenberg, F. and Buschiazzo, A. (2017). Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis. Acta Crystallogr F Struct Biol Commun 73(Pt 3): 123-129.
  2. Trajtenberg, F., Imelio, J. A., Machado, M. R., Larrieux, N., Marti, M. A., Obal, G., Mechaly, A. E. and Buschiazzo, A. (2016). Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action. Elife 5.
  3. Moran-Barrio, J., Lisa, M. N., Larrieux, N., Drusin, S. I., Viale, A. M., Moreno, D. M., Buschiazzo, A. and Vila, A. J. (2016). Crystal Structure of the Metallo-beta-Lactamase GOB in the Periplasmic Dizinc Form Reveals an Unusual Metal Site. Antimicrob Agents Chemother 60(10): 6013-6022.
  4. Obal, G., Trajtenberg, F., Carrion, F., Tome, L., Larrieux, N., Zhang, X., Pritsch, O. and Buschiazzo, A. (2015). STRUCTURAL VIROLOGY. Conformational plasticity of a native retroviral capsid revealed by x-ray crystallography. Science 349(6243): 95-98.
  5. Trajtenberg, F., Albanesi, D., Ruétalo, N., Botti, H., Mechaly, A. E., Nieves, M., Aguilar, P. S., Cybulski, L., Larrieux, N., de Mendoza, D. and Buschiazzo, A. (2014). Allosteric activation of bacterial response regulators: the role of the cognate histidine kinase beyond phosphorylation. MBio 5(6): e02105. 
  6. Trajtenberg, F., Altabe, S., Larrieux, N., Ficarra, F., de Mendoza, D., Buschiazzo, A. and Schujman, G. E. (2014). Structural insights into bacterial resistance to cerulenin. FEBS J 281(10): 2324-2338.
  7. Martinez, A., Peluffo, G., Petruk, A. A., Hugo, M., Pineyro, D., Demicheli, V., Moreno, D. M., Lima, A., Batthyany, C., Duran, R., Robello, C., Marti, M. A., Larrieux, N., Buschiazzo, A., Trujillo, M., Radi, R. and Piacenza, L. (2014). Structural and molecular basis of the peroxynitrite-mediated nitration and inactivation of Trypanosoma cruzi iron-superoxide dismutases (Fe-SODs) A and B: disparate susceptibilities due to the repair of Tyr35 radical by Cys83 in Fe-SODB through intramolecular electron transfer. J Biol Chem 289(18): 12760-12778.
  8. Buschiazzo, A., Muia, R., Larrieux, N., Pitcovsky, T., Mucci, J. and Campetella, O. (2012). Trypanosoma cruzi trans-sialidase in complex with a neutralizing antibody: structure/function studies towards the rational design of inhibitors. PLoS Pathog 8(1): e1002474.
  9. Ortiz, C., Larrieux, N., Medeiros, A., Botti, H., Comini, M. and Buschiazzo, A. (2011). Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate. Acta Crystallogr Sect F Struct Biol Cryst Commun 67(Pt 11): 1457-1461.
1 Protocol published
Snapshots of the Signaling Complex DesK:DesR in Different Functional States Using Rational Mutagenesis and X-ray Crystallography
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli ...