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Coauthors
Gregory Davis 1 protocol

Yanfang Jiang 1 protocol

Timothy Seebeck 1 protocol

Yongmei Feng 1 protocol

Fuqiang Chen
  • Genome Engineering, MilliporeSigma, USA
Contributions
  • 1 Author merit

Education

Ph. D., Oregon State University, USA, 1991

Current position

Principal Scientist, Genome Engineering, MilliporeSigma, USA

Publications

  1. Chen, F. Q., Xaio, D., Feng, Y. M., Seebeck, T., Jiang, Y. F. and Davis, G. D. (2017). Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR (proxy-CRISPR) targeting. Nat Commun 8 (14958) doi:10.1038.
  2. Chen, F., Pruett-Miller, S. M. and Davis, G. D. (2015). Gene editing using ssODNs with engineered endonucleases. Methods Mol Biol 1239: 251-265.
  3. Chen, F., Pruett-Miller, S. M., Huang, Y., Gjoka, M., Duda, K., Taunton, J., Collingwood, T. N., Frodin, M. and Davis, G. D. (2011). High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases. Nat Methods 8(9): 753-755.
  4. Raina, S., Mahalingam, R., Chen, F. and Fedoroff, N. (2002). A collection of sequenced and mapped Ds transposon insertion sites in Arabidopsis thaliana. Plant Mol Biol 50(1): 93-110.
  5. Foolad, M. R., Chen, F. Q. and Lin, G. Y. (1999). RFLP mapping of QTLs conferring salt tolerance during vegetative growth in an interspecific cross of tomato. Theor Appl Genet 99: 235-243.
  6. Foolad, M. R., Lin, G.Y. and Chen, F. Q. (1999). Comparison of QTLs for seed germination under non-stress, cold stress and salt stress in tomato. Plant Breeding 118:167-173.
  7. Chen, F. Q., Foolad, M. R., Hyman, J. R., Clair, D. A. St. and Beelman, R. (1999). Mapping of QTLs for lycopene and other fruit traits in a Lycopersicon esculentum and L. pimpinellifolium cross and comparison of QTLs across tomato species. Molecular Breeding 5:283-299.
  8. Chen, F. Q. and Foolad, M. R. (1999). Nucellar cell-specific expression of a lipid transfer protein (LTP) gene in barley (Hordeum vulgare L.). Plant Cell Rep 18:445-450.
  9. Chen, F. Q. and Foolad, M. R. (1999). A molecular linkage map of tomato based on a cross between Lycopersicon esculentum and L. pimpinellifolium and its comparison with other molecular maps of tomato. Genome 42:94-103.
  10. Foolad, M. R., Chen, F. Q. and Lin, G. Y. (1998). RFLP mapping of QTLs conferring cold tolerance during germination in an interspecific cross of tomato. Molecular Breeding 4:519-529.
  11. Foolad, M. R., Chen, F. Q. and Lin, G. Y. (1998). RFLP mapping of QTLs conferring salt tolerance during germination in an interspecific cross of tomato. Theor Appl Genet 97:1133-1144.
  12. Foolad, M .R., and Chen, F. Q. (1998). RAPD markers associated with salt tolerance in an interspecific cross of tomato (Lycopersicon esculentum and L. pennellii). Plant Cell Rep 17:306-312.
  13. Chen, F. and Foolad, M. R. (1997). Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration. Plant Mol Biol 35(6): 821-831.
  14. Oziel, A., Hayes, P. M, Chen, F. Q. and Jones, B. (1996). Application of quantitative trait locus mapping to the development of winter habit malting barley. Plant Breeding 115: 43-51.
  15. Hayes, P., Prehn, D., Vivar, H., Blake, T., Comeau, A., Henry, I., Johnston, M., Jones, B., Steffenson, B., Pierre, C. A. St. and Chen, F. Q. (1996). Multiple disease resistance loci and their relationship to agronomic and quality loci in a spring barley population. Journal of Agricultural Genomics 1-9.
  16. van Zee, K., Chen, F. Q., Hayes, P. M., Close, T. and Chen, T. H. H. (1995). Cold-specific induction of a subset of dehydrin gene family members in barley (Hordeum vulgare L.). Plant Physiol 108: 1233-1239.
  17. Chen, F., Hayes, P. M., Mulrooney, D. M. and Pan, A. (1994). Identification and characterization of cDNA clones encoding plant calreticulin in barley. Plant Cell 6(6): 835-843.
  18. Chen, F. Q., Prehn, D., Hayes, P. M., Mulrooney, D., Corey, A. and Vivar, H. (1994). Mapping genes for resistance to barley stripe rust (Puccinia striiformis f. sp. hordei). Theor Appl Genet 88(2): 215-219.
  19. Chen, F., Hayes, P. M., Mulrooney, D. M. and Pan, A. (1994). Nucleotide sequence of a cDNA encoding a heat-shock protein (HSP70) from barley (Hordeum vulgare L.). Plant Physiol 106(2): 815.
  20. Chen, F. and Hayes, P. M. (1994). Nucleotide sequence and developmental expression of duplicated genes encoding protein disulfide isomerase in barley (Hordeum vulgare L.). Plant Physiol 106(4): 1705-1706.
  21. Pan, A., Hayes, P. M., Chen, F., Chen, T. H., Blake, T., Wright, S., Karsai, I. and Bedo, Z. (1994). Genetic analysis of the components of winterhardiness in barley (Hordeum vulgare L.). Theor Appl Genet 89(7-8): 900-910.
  22. Van Zee, K., Chen, F. Q., Hayes, P. M. and Chen, T. H. H. (1994). Low temperature induction of dehydrins in barley (Hordeum vulgare L.). In: Dorffling, K., Brettschneider, B., Tantau, H. and Pithan, K. (Eds.). Agriculture Crop Adaptation To Cool Climates. ECSP-EEC-EAEC, Brussels, pp 317-324.
  23. Kleinhofs, A., Kilian, A., Saghai Maroof, M. A., Biyashev, R. M., Hayes, P., Chen, F. Q., Lapitan, N., Fenwick, A., Blake, T. K., Kanazin, V., Ananiev, E., Dahleen, L., Kudrna, D., Bollinger, J., Knapp, S. J., Liu, B., Sorrells, M., Heun, M., Franckowiak, J. D., Hoffman, D., Skadsen, R. and Steffenson, B. J. (1993). A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86(6): 705-712.
  24. Hayes, P. M., Liu, B. H., Knapp, S. J., Chen, F., Jones, B., Blake, T., Franckowiak, J., Rasmusson, D., Sorrells, M., Ullrich, S. E., Wesenberg, D. and Kleinhofs, A. (1993). Quantitative trait locus effects and environmental interaction in a sample of North American barley germ plasm. Theor Appl Genet 87(3): 392-401.
  25. Hayes, P. M., Blake, T., Chen, T. H., Tragoonrung, S., Chen, F., Pan, A. and Liu, B. (1993). Quantitative trait loci on barley (Hordeum vulgare L.) chromosome 7 associated with components of winterhardiness. Genome 36(1): 66-71.
  26. Chen, F. Q. and Hayes, P. M. (1992). Genetic basis of seed set in barley genotypes varying in compatibility with Hordeum bulbosum. Genome 35:799-805.
  27. Chen, F. Q. and Hayes, P. M. (1991). Effect of exogenous plant growth regulators on in vitro seed growth, embryo development, and haploid production in a cross between H. vulgare (L.) and H. bulbosum (L.) Plant Cell, Tissue and Organ Culture 26: 179-184.
  28. Chen, F. Q., Hayes, P. M. and Rivin, C. (1991). Wide hybridization of Hordeum vulgare (L.) X Zea mays (L.). Genome 34:603-605.
  29. Chen, F. Q. and Hayes, P. M. (1989). A comparison of Hordeum bulbosum-mediated haploid production efficiency in barley using in vitro floret and tiller culture. Theor Appl Genet 77(5): 701-704.
  30. Hayes, P. M. and Chen, F. Q. (1989). Genotypic variation for Hordeum bulbosum-mediated haploid production in winter and facultative barley. Crop Sci 29: 1184-1188.
1 Protocol published
Improving CRISPR Gene Editing Efficiency by Proximal dCas9 Targeting
Authors:  Fuqiang Chen, Xiao Ding, Yongmei Feng, Timothy Seebeck, Yanfang Jiang and Gregory D Davis, date: 08/05/2017, view: 925, Q&A: 0
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems function as an adaptive immune system in bacteria and archaea for defense against invading viruses and plasmids (Barrangou and Marraffini, 2014). ...