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Coauthors
Eugene I Shakhnovich Department of Chemistry and Chemical Biology, Harvard University, USA
1 protocol

Shimon Bershtein Department of Life Sciences, Ben-Gurion University of the Negev, Israel
1 protocol

Sanchari Bhattacharyya
  • Department of Chemistry and Chemical Biology, Harvard University, USA
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  • 1 Author merit
1 Protocol published
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:  Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich, date: 07/20/2017, view: 522, Q&A: 0
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...