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Coauthors
Jun Lu Department of Genetics, Yale University School of Medicine, USA
1 protocol

Wen Pan State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine
2 protocols

Jijun Cheng
  • Department of Genetics, Yale University School of Medicine, USA
Contributions
  • 1 Author merit

Education

Ph.D, University of Kentucky, US, 2000

Current position

Associate Research Scientist, Genetics, Yale University, US

Publications

  1. Liu, J., Guo, B., Chen, Z., Wang, N., Iacovino, M., Cheng, J., Roden, C., Pan, W., Khan, S., Chen, S., Kyba, M., Fan, R., Guo, S. and Lu, J. (2017). miR-125b promotes MLL-AF9-driven murine acute myeloid leukemia involving a VEGFA-mediated non-cell-intrinsic mechanism. Blood 129(11): 1491-1502.
  2. Roden, C., Gaillard, J., Kanoria, S., Rennie, W., Barish, S., Cheng, J., Pan, W., Liu, J., Cotsapas, C., Ding, Y. and Lu, J. (2017). Novel determinants of mammalian primary microRNA processing revealed by systematic evaluation of hairpin-containing transcripts and human genetic variation. Genome Res 27(3): 374-384.
  3. Hu, Z., Xu, J., Cheng, J., McMichael, E., Yu, L. and Carson, W. E., 3rd (2017). Targeting tissue factor as a novel therapeutic oncotarget for eradication of cancer stem cells isolated from tumor cell lines, tumor xenografts and patients of breast, lung and ovarian cancer. Oncotarget 8(1): 1481-1494.
  4. Hu, Z., Cheng, J., Xu, J., Ruf, W. and Lockwood, C. J. (2017). Tissue factor is an angiogenic-specific receptor for factor VII-targeted immunotherapy and photodynamic therapy. Angiogenesis 20(1): 85-96.
  5. Cheng, J., Pan, W., Roden, C., Chen, Z. and Lu, J. (2016). Molecular Chipper: Functional Mapping of the Non-Coding Genome with CRISPR. Next Generat Sequenc & Applic 3:132.
  6. Hysolli, E., Tanaka, Y., Su, J., Kim, K. Y., Zhong, T., Janknecht, R., Zhou, X. L., Geng, L., Qiu, C., Pan, X., Jung, Y. W., Cheng, J., Lu, J., Zhong, M., Weissman, S. M. and Park, I. H. (2016). Regulation of the DNA Methylation Landscape in Human Somatic Cell Reprogramming by the miR-29 Family. Stem Cell Reports 7(1): 43-54.
  7. Cheng, J., Roden, C. A., Pan, W., Zhu, S., Baccei, A., Pan, X., Jiang, T., Kluger, Y., Weissman, S. M., Guo, S., Flavell, R. A., Ding, Y. and Lu, J. (2016). A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions. Nat Commun 7: 11178.
  8. Xiang, Y., Cheng, J., Wang, D., Hu, X., Xie, Y., Stitham, J., Atteya, G., Du, J., Tang, W. H., Lee, S. H., Leslie, K., Spollett, G., Liu, Z., Herzog, E., Herzog, R. I., Lu, J., Martin, K. A. and Hwa, J. (2015). Hyperglycemia repression of miR-24 coordinately upregulates endothelial cell expression and secretion of von Willebrand factor. Blood 125(22): 3377-3387.
  9. Zhang, P. X., Cheng, J., Zou, S., D'Souza, A. D., Koff, J. L., Lu, J., Lee, P. J., Krause, D. S., Egan, M. E. and Bruscia, E. M. (2015). Pharmacological modulation of the AKT/microRNA-199a-5p/CAV1 pathway ameliorates cystic fibrosis lung hyper-inflammation. Nat Commun 6: 6221.
  10. Liu, C., Rennie, W. A., Carmack, C. S., Kanoria, S., Cheng, J., Lu, J. and Ding, Y. (2014). Effects of genetic variations on microRNA: target interactions. Nucleic Acids Res 42(15): 9543-9552.
  11. Guo, S., Zi, X., Schulz, V. P., Cheng, J., Zhong, M., Koochaki, S. H., Megyola, C. M., Pan, X., Heydari, K., Weissman, S. M., Gallagher, P. G., Krause, D. S., Fan, R. and Lu, J. (2014). Nonstochastic reprogramming from a privileged somatic cell state. Cell 156(4): 649-662.
  12. Cheng, J., Guo, S., Chen, S., Mastriano, S. J., Liu, C., D'Alessio, A. C., Hysolli, E., Guo, Y., Yao, H., Megyola, C. M., Li, D., Liu, J., Pan, W., Roden, C. A., Zhou, X. L., Heydari, K., Chen, J., Park, I. H., Ding, Y., Zhang, Y. and Lu, J. (2013). An extensive network of TET2-targeting MicroRNAs regulates malignant hematopoiesis. Cell Rep 5(2): 471-481.
  13. Megyola, C. M., Gao, Y., Teixeira, A. M., Cheng, J., Heydari, K., Cheng, E. C., Nottoli, T., Krause, D. S., Lu, J. and Guo, S. (2013). Dynamic migration and cell-cell interactions of early reprogramming revealed by high-resolution time-lapse imaging. Stem Cells 31(5): 895-905.
  14. Adams, B. D., Guo, S., Bai, H., Guo, Y., Megyola, C. M., Cheng, J., Heydari, K., Xiao, C., Reddy, E. P. and Lu, J. (2012). An in vivo functional screen uncovers miR-150-mediated regulation of hematopoietic injury response. Cell Rep 2(4): 1048-1060.
  15. Cheng, J., Xu, J., Duanmu, J., Zhou, H., Booth, C. J. and Hu, Z. (2011). Effective treatment of human lung cancer by targeting tissue factor with a factor VII-targeted photodynamic therapy. Curr Cancer Drug Targets 11(9): 1069-1081.
  16. Duanmu, J., Cheng, J., Xu, J., Booth, C. J. and Hu, Z. (2011). Effective treatment of chemoresistant breast cancer in vitro and in vivo by a factor VII-targeted photodynamic therapy. Br J Cancer 104(9): 1401-1409.
  17. Agarwal, A., Louise-May, S., Thanassi, J. A., Podos, S. D., Cheng, J., Thoma, C., Liu, C., Wiles, J. A., Nelson, D. M., Phadke, A. S., Bradbury, B. J., Deshpande, M. S. and Pucci, M. J. (2007). Small molecule inhibitors of E. coli primase, a novel bacterial target. Bioorg Med Chem Lett 17(10): 2807-2810.
  18. Cheng, J., Thanassi, J. A., Thoma, C. L., Bradbury, B. J., Deshpande, M. and Pucci, M. J. (2007). Dual targeting of DNA gyrase and topoisomerase IV: target interactions of heteroaryl isothiazolones in Staphylococcus aureus. Antimicrob Agents Chemother 51(7): 2445-2453.
  19. Pucci, M. J., Cheng, J., Podos, S. D., Thoma, C. L., Thanassi, J. A., Buechter, D. D., Mushtaq, G., Vigliotti, G. A., Jr., Bradbury, B. J. and Deshpande, M. (2007). In vitro and in vivo antibacterial activities of heteroaryl isothiazolones against resistant gram-positive pathogens. Antimicrob Agents Chemother 51(4): 1259-1267.
  20. Wang, Q., Lucien, E., Hashimoto, A., Pais, G. C., Nelson, D. M., Song, Y., Thanassi, J. A., Marlor, C. W., Thoma, C. L., Cheng, J., Podos, S. D., Ou, Y., Deshpande, M., Pucci, M. J., Buechter, D. D., Bradbury, B. J. and Wiles, J. A. (2007). Isothiazoloquinolones with enhanced antistaphylococcal activities against multidrug-resistant strains: effects of structural modifications at the 6-, 7-, and 8-positions. J Med Chem 50(2): 199-210.
  21. Wiles, J. A., Song, Y., Wang, Q., Lucien, E., Hashimoto, A., Cheng, J., Marlor, C. W., Ou, Y., Podos, S. D., Thanassi, J. A., Thoma, C. L., Deshpande, M., Pucci, M. J. and Bradbury, B. J. (2006). Biological evaluation of isothiazoloquinolones containing aromatic heterocycles at the 7-position: In vitro activity of a series of potent antibacterial agents that are effective against methicillin-resistant Staphylococcus aureus. Bioorg Med Chem Lett 16(5): 1277-1281.
  22. Wiles, J. A., Wang, Q., Lucien, E., Hashimoto, A., Song, Y., Cheng, J., Marlor, C. W., Ou, Y., Podos, S. D., Thanassi, J. A., Thoma, C. L., Deshpande, M., Pucci, M. J. and Bradbury, B. J. (2006). Isothiazoloquinolones containing functionalized aromatic hydrocarbons at the 7-position: synthesis and in vitro activity of a series of potent antibacterial agents with diminished cytotoxicity in human cells. Bioorg Med Chem Lett 16(5): 1272-1276.
  23. Wiles, J. A., Hashimoto, A., Thanassi, J. A., Cheng, J., Incarvito, C. D., Deshpande, M., Pucci, M. J. and Bradbury, B. J. (2006). Isothiazolopyridones: synthesis, structure, and biological activity of a new class of antibacterial agents. J Med Chem 49(1): 39-42.
  24. Cheng, J., Park, T. S., Chio, L. C., Fischl, A. S. and Ye, X. S. (2003). Induction of apoptosis by sphingoid long-chain bases in Aspergillus nidulans. Mol Cell Biol 23(1): 163-177.
  25. Cheng, J., Park, T. S., Fischl, A. S. and Ye, X. S. (2001). Cell cycle progression and cell polarity require sphingolipid biosynthesis in Aspergillus nidulans. Mol Cell Biol 21(18): 6198-6209.
  26. Lies, C. M., Cheng, J., James, S. W., Morris, N. R., O'Connell, M. J. and Mirabito, P. M. (1998). BIMAAPC3, a component of the Aspergillus anaphase promoting complex/cyclosome, is required for a G2 checkpoint blocking entry into mitosis in the absence of NIMA function. J Cell Sci 111 ( Pt 10): 1453-1465.
  27. Dong, S., Fu, C., Cheng, J. and Gao, M. (2000). Genetic control by allozymes in Artemia. Periodical of Ocean University of China 02 :102-108.
  28. Gao, M., Cheng, J. and Cai, Y. (1995). Functional study on rare males in parthenogenesis Artemia. Chinese Science Bulletin 15: 2276-2781.
1 Protocol published
Dense sgRNA Library Construction Using a Molecular Chipper Approach
Authors:  Jijun Cheng, Wen Pan and Jun Lu, date: 06/20/2017, view: 808, Q&A: 0
Genetic screens using single-guide-RNA (sgRNA) libraries and CRISPR technology have been powerful to identify genetic regulators for both coding and noncoding regions of the genome. Interrogating functional elements in noncoding regions requires ...