Search
Coauthors
Tiina Alamäe Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Kristina Põšnograjeva Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Triinu Visnapuu Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Katrin Viigand Institute of Molecular and Cell Biology, University of Tartu, Estonia
1 protocol

Karin Ernits
  • Institute of Molecular and Cell Biology, University of Tartu, Estonia
Contributions
  • 1 Author merit

Education

Master, University of Tartu, Estonia, 2011

Current position

Doctoral student, Institute of Molecular and Cell Biology, University of Tartu, Éstonia

Publications

  1. Mardo, K., Visnapuu, T., Vija, H., Aasamets, A., Viigand, K. and Alamae, T. (2017). A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. PLoS One 12(1): e0169989.
  2. Viigand, K., Visnapuu, T., Mardo, K., Aasamets, A. and Alamae, T. (2016). Maltase protein of Ogataea (Hansenula) polymorpha is a counterpart to the resurrected ancestor protein ancMALS of yeast maltases and isomaltases. Yeast 33(8): 415-432.
  3. Visnapuu, T., Mardo, K. and Alamae, T. (2015). Levansucrases of a Pseudomonas syringae pathovar as catalysts for the synthesis of potentially prebiotic oligo- and polysaccharides. N Biotechnol 32(6): 597-605.
  4. Mardo, K., Visnapuu, T., Gromkova, M., Aasamets, A., Viigand, K., Vija, H. and Alamae, T. (2014). High-throughput assay of levansucrase variants in search of feasible catalysts for the synthesis of fructooligosaccharides and levan. Molecules 19(6): 8434-8455.
  5. Mardo, K., Visnapuu, T., Vija, H., Elmi, T. and Alamae, T. (2014). Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc-3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000. Biotechnol Appl Biochem 61(1): 11-22.
  6. Alvarez, Y., Esteban-Torres, M., Cortes-Cabrera, A., Gago, F., Acebron, I., Benavente, R., Mardo, K., de Las Rivas, B., Munoz, R. and Mancheno, J. M. (2014). Esterase LpEst1 from Lactobacillus plantarum: a novel and atypical member of the alphabeta hydrolase superfamily of enzymes. PLoS One 9(3): e92257.
  7. Mardo, K., Mardo, K., Visnapuu, T. and Alamäe, T. (2014). Comparison of catalytic properties of levansucrases Lsc2 and Lsc3 of Pseudomonas syringae pv. tomato. In: Copikova, J. and Šarka, E. (Eds). Proceedings of the 10th International Conference on Polysaccharides-Glycoscience: 10th International Conference on Polysaccharides-Glycoscience. Prague, Czech Republic, 111–113.
  8. Mardo, K., Mardo, K., Visnapuu, T., Gromkova, M. and Alamäe, T. (2014). Experimental verification of catalytic triad residues of Pseudomonas syringae pv. tomato levansucrase Lsc2. In: Copikova, J. and Šarka, E. (Eds). Proceedings of the 10th International Conference on Polysaccharides-Glycoscience: 10th International Conference on Polysaccharides-Glycoscience. Prague, Czech Republic, 84–87.
  9. Alamäe, T., Visnapuu, T., Mardo, K., Mäe, A.  Zamfir, A. D. (2012). Levansucrases of Pseudomonas bacteria: novel approaches for protein expression, assay of enzymes, fructooligosaccharides and heterooligofructans. In:  Rauter, A. P. and Lindhorst, T. K. (Eds). Carbohydrate Chemistry. Cambridge, UK: Royal Soc Chemistry, 176–191.
  10. Mardo, K., Mäe, A., Visnapuu, T. and Alamäe, T. (2012). Different tagging modes of a levansucrase protein from Pseudomonas syringae pv. tomato for structure-function analysis. In: Rapkova, R., Copikova, J. and Šarka, E. (Eds). Proceedings of the 8th International Conference on Polysaccharides-Glycoscience: 8th International Conference on Polysaccharides-Glycoscience. Prague, Czech Republic: Czech Chemical Society, 99–102.
  11. Visnapuu, T., Mardo, K., Mosoarca, C., Zamfir, A. D., Vigants, A. and Alamae, T. (2011). Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: substrate specificity, polymerizing properties and usage of different acceptors for fructosylation. J Biotechnol 155(3): 338-349.
  12. Mardo, K., Visnapuu, T. and Alamäe, T. (2010). Isolation and high-throughput screening methods of levansucrase mutants of a plant pathogen Pseudomonas syringae DC3000. In: (Eds) In: Rapkova, R., Copikova, J. and Šarka, E. (Eds). Proceedings of the 8th International Conference on Polysaccharides-Glycoscience: 8th International Conference on Polysaccharides-Glycoscience. Prague, Czech Republic: Czech Chemical Society, 36–39.
1 Protocol published
Thermostability Measurement of an α-Glucosidase Using a Classical Activity-based Assay and a Novel Thermofluor Method
α-glucosidases (including maltases and isomaltases) are enzymes which release glucose from a set of α-glucosidic substrates. Their catalytic activity, substrate specificity and thermostability can be assayed using this trait. Thermostability of ...