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Coauthors
David Pincus Whitehead Institute for Biomedical Research, USA
1 protocol

Xu Zheng
  • Whitehead Institute for Biomedical Research, USA
  • 1 Author merit
1 Protocol published
Serial Immunoprecipitation of 3xFLAG/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins
Authors:  Xu Zheng and David Pincus, date: 06/20/2017, view: 1119, Q&A: 0
This method was generated to isolate high affinity protein complexes from yeast lysate by performing serial affinity purification of doubly tagged 3xFLAG/V5 proteins. First, the bait protein of interest is immunoprecipitated by anti-FLAG-conjugated ...