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Coauthors
Ching-Fu Tu Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
1 protocol

Chin-kai Chuang Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
1 protocol

Chien-Hong Chen
  • Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
Contributions
  • 1 Author merit

Education

Ph.D, Institute of Biotechnology/Taiwan University, Republic of China, 2012

Current position

Research Embryologist, Reproductive Medicine Center, Lee Women's Hospital, Republic of China

Publications (Since 2008)

  1. Chuang, C. K., Chen, C. H., Huang, C. L., Su, Y. H., Peng, S. H., Lin, T. Y., Tai, H. C., Yang, T. S. and Tu, C. F. (2016). Generation of GGTA1 Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors. Anim Biotechnol: 1-8.
  2. Chen, C. H., Su, Y. H., Lee, K. H. and Chuang, C. K. (2016). Germline Competent Pluripotent Mouse Stem Cells Generated by Plasmid Vectors. Anim Biotechnol 27(3): 157-165.
  3. Intawicha, P., Siriboon, C., Chen, C. H., Chiu, Y. T., Lin, T. A., Kere, M., Lo, N. W., Lee, K. H., Chang, L. Y., Chiang, H. I. and Ju, J. C. (2016). Derivation and characterization of putative embryonic stem cells from cloned rabbit embryos. Theriogenology 86(7): 1799-1810.
  4. Du, F., Chen, C. H., Li, Y., Hu, Y., An, L. Y., Yang, L., Zhang, J., Chen, Y. E. and Xu, J. (2015). Derivation of Rabbit Embryonic Stem Cells from Vitrified-Thawed Embryos. Cell Reprogram 17(6): 453-462.
  5. Lo, N. W., Intawicha, P., Chiu, Y. T., Lee, K. H., Lu, H. C., Chen, C. H., Chang, Y. H., Chen, C. D. and Ju, J. C. (2015). Leukemia inhibitory factor and fibroblast growth factor 2 critically and mutually sustain pluripotency of rabbit embryonic stem cells. Cell Transplant 24(3): 319-338.
  6. Siriboon, C., Lin, Y. H., Kere, M., Chen, C. D., Chen, L. R., Chen, C. H., Tu, C. F., Lo, N. W. and Ju, J. C. (2015). Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning. PLoS One 10(2): e0118165.
  7. Tseng, J. K., Lo, N. W., Shen, P. C., Chiang, H. I., Fan, Y. K., Chen, C. H. and Ju, J. C. (2014). Physiologic Responses of Mammalian Oocytes and Embryos under Thermal Stress: An Overview. Adaptive Medicine 6: 49-56.
  8. Chen, C. H., Jiang, B. H., Huang, S. Y., Yang, T. S., Lee, K. H., Tu, C. F. and Wu, S. C. (2013). Genetic polymorphisms, growth performance, hematological parameters, serum enzymes, and reproductive characteristics in phenotypically normal Landrace boars produced by somatic cell nuclear transfer. Theriogenology 80(9): 1088-1096.
  9. Chen, C. H., Du, F., Xu, J., Chang, W. F., Liu, C. C., Su, H. Y., Lin, T. A., Ju, J. C., Cheng, W. T., Wu, S. C., Chen, Y. E. and Sung, L. Y. (2013). Synergistic effect of trichostatin A and scriptaid on the development of cloned rabbit embryos. Theriogenology 79(9): 1284-1293.
  10. Lei, L., Li, L., Du, F., Chen, C. H., Wang, H. and Keefer, C. L. (2013). Monitoring bovine fetal fibroblast reprogramming utilizing a bovine NANOG promoter-driven EGFP reporter system. Mol Reprod Dev 80(3): 193-203.
  11. Chen, C. H., Xu, J., Chang, W. F., Liu, C. C., Su, H. Y., Chen, Y. E., Du, F. and Sung, L. Y. (2012). Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos. Reprod Biomed Online 25(4): 358-370.
  12. Xue, F., Ma, Y., Chen, Y. E., Zhang, J., Lin, T. A., Chen, C. H., Lin, W. W., Roach, M., Ju, J. C., Yang, L., Du, F. and Xu, J. (2012). Recombinant rabbit leukemia inhibitory factor and rabbit embryonic fibroblasts support the derivation and maintenance of rabbit embryonic stem cells. Cell Reprogram 14(4): 364-376.
  13. Chen, C. H., Chang, W. F., Liu, C. C., Su, H. Y., Shyue, S. K., Cheng, W. T., Chen, Y. E., Wu, S. C., Du, F., Sung, L. Y. and Xu, J. (2012). Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos. Reprod Biomed Online 24(4): 433-442.
  14. Sung, L. Y., Chen, C. H., Xu, J., Lin, T. A., Su, H. Y., Chang, W. F., Liu, C. C., Sung, Y. S., Cheng, W. T., Zhang, J., Tian, X. C., Ju, J. C., Chen, Y. E., Wu, S. C. and Du, F. (2011). Follicular oocytes better support development in rabbit cloning than oviductal oocytes. Cell Reprogram 13(6): 503-512.
  15. Lin, T. A., Chen, C. H., Sung, L. Y., Carter, M. G., Chen, Y. E., Du, F., Ju, J. C. and Xu, J. (2011). Open-pulled straw vitrification differentiates cryotolerance of in vitro cultured rabbit embryos at the eight-cell stage. Theriogenology 75(4): 760-768.
  16. Kao, C. F., Chuang, C. Y., Chen, C. H. and Kuo, H. C. (2008). Human pluripotent stem cells: current status and future perspectives. Chin J Physiol 51(4): 214-225.
  17. Tu, C. F., Tai, H. C., Chen, C. M., Huang, T. T., Lee, J. M., Yang, T. S., Chen, C. H., Tseng, Y. L., Chou, N. K. and Lee, P. H. (2008). Human leukocyte antigen-DR matching improved skin graft survival from transgenic pigs to accommodate SCID mice reconstituted with human peripheral blood mononuclear cells. Transplant Proc 40(2): 578-580.
  18. Chen, C. H., Stone, L., Ju, J. C., Lien, W. T., Liu, M. S., Tu, C. F. and Lee, K. H. (2008). Transgenic cloned mice expressing enhanced green fluorescent protein generated by activation stimuli combined with 6-dimethylaminopurine. Reprod Domest Anim 43(5): 547-555.
  19. Lin, T. A., Tsay, C., Chen, C. H., Tang, P. C. and Ju, J. C. (2008). Nuclear and cytoskeletal dynamics during oocyte maturation and development of somatic cell cloned pig embryos injected with membrane disintegrated donor cells. Anim Reprod Sci 103(1-2): 107-119.
1 Protocol published
Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors
Authors:  Chin-kai Chuang, Ching-Fu Tu and Chien-Hong Chen, date: 06/05/2017, view: 1038, Q&A: 0
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription ...