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Coauthors
Chien-Hong Chen Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
1 protocol

Ching-Fu Tu Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
1 protocol

Chin-kai Chuang
  • Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
  • 1 Author merit

Education

Ph.D in Life Science, Institute of Life Science, National Tsing Hua University, Taiwan, 1989

Current position

Researcher, Division of Animal Technology, Laboratories of Animal Technology, Agricultural Technology Research Institute, Taiwan

Publications (Since 2005)

  1. Chuang, C. K., Chen, C. H., Huang, C. L., Su, Y. H., Peng, S. H., Lin, T. Y., Tai, H. C., Yang, T. S. and Tu, C. F. (2016). Generation of GGTA1 Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors. Anim Biotechnol: 1-8.
  2. Chen, C. H., Su, Y. H., Lee, K. H. and Chuang, C. K. (2016). Germline Competent Pluripotent Mouse Stem Cells Generated by Plasmid Vectors. Anim Biotechnol 27(3): 157-165.
  3. Chuang, C. K. and Lin, T. Y. (2016). PTD-J-X: To elicit humoral and cellular immunity as will. Immunother Open Acc 2:129.
  4. Liu, P. C., Chen, C. A., Yen, C. H., Lee, M. H., Chuang, C. K., Tu, C. F. and Su, B. L. (2016). Application of xenogenic anti-canine distemper virus antibodies in treatment of canine distemper puppies. J Small Anim Pract 57(11): 626-630.
  5. Lin, T. Y., Su, Y. H. and Chuang, C. K. (2015). PTD-J Domain Acts As an Antigen Carrier for Cell Mediated Immunity. J Immunotherapy Application 2: 1.
  6. Su, Y. H., Lin, T. Y., Huang, C. L., Tu, C. F. and Chuang, C. K. (2015). Construction of a CRISPR-Cas9 System for Pig Genome Targeting. Anim Biotechnol 26(4): 279-288.
  7. Lin, T. Y., Su, Y. H., Lee, K. H. and Chuang, C. K. (2015). The J-domain of heat shock protein 40 can enhance the transduction efficiency of arginine-rich cell-penetrating peptides. BioMed Res Int 698067.
  8. Lin, T. Y., Su, Y. S., Lin, T. Y, Hung, L. Y. and Chuang, C. K. (2013). Hsp70 Protein Can Enhance the Immunogenicity of FMDV VP1 Epitopes Fused with PTD-J Domain. Scholarly J Biol Sci 2(4): 39-48.
  9. Lee, K. H., Sun, J. C., Chuang, C. K., Guo, S. F., Tu, C. F. and Ju, J. C. (2013). An efficient and mass reproducible method for vitrifying mouse embryos on a paper in cryotubes. Cryobiology 66(3): 311-317.
  10. Lee, K. H., Chuang, C. K., Guo, S. F. and Tu, C. F. (2012). Simple and efficient derivation of mouse embryonic stem cell lines using differentiation inhibitors or proliferation stimulators. Stem Cells Dev 21(3): 373-383.
  11. Tsou, J. H., Chang, K. C., Chang-Liao, P. Y., Yang, S. T., Lee, C. T., Chen, Y. P., Lee, Y. C., Lin, B. W., Lee, J. C., Shen, M. R., Chuang, C. K., Chang, W. C., Wang, J. M. and Hung, L. Y. (2011). Aberrantly expressed AURKC enhances the transformation and tumourigenicity of epithelial cells. J Pathol 225(2): 243-254.
  12. Huang, S. Y., Lin, J. H., Teng, S. H., Sun, H. S., Chen, Y. H., Chen, H. H., Liao, J. Y., Chung, M. T., Chen, M. Y., Chuang, C. K., Lin, E. C. and Huang, M. C. (2011). Differential expression of porcine testis proteins during postnatal development. Anim Reprod Sci 123(3-4): 221-233.
  13. Chuang, C. K., Su, Y. S., Fan, C. T., Lee, W. C. and Chen, M. Y. (2009). A dual-functional E. coli vector for expressing recombinant protein with high solubility and antigen presentation ability. Protein Expr Purif 65(1): 51-56.
  14. Li, H. F., Huang, C. H., Ai, L. S., Chuang, C. K. and Chen, S. S. (2009). Mutagenesis of the fusion peptide-like domain of hepatitis C virus E1 glycoprotein: involvement in cell fusion and virus entry. J Biomed Sci 16: 89.
  15. Chuang, C. K., Lee, K. H., Fan, C. T. and Su, Y. S. (2009). Porcine type III RNA polymerase III promoters for short hairpin RNA expression. Anim Biotechnol 20(1): 34-39.
  16. Chen, S. S., Yang, P., Ke, P. Y., Li, H. F., Chan, W. E., Chang, D. K., Chuang, C. K., Tsai, Y. and Huang, S. C. (2009). Identification of the LWYIK motif located in the human immunodeficiency virus type 1 transmembrane gp41 protein as a distinct determinant for viral infection. J Virol 83(2): 870-883.
  17. Gau, B. H., Chu, I. M., Huang, M. C., Yang, K. T., Chiou, S. H., Fan, Y. H., Chen, M. Y., Lin, J. H., Chuang, C. K., Huang, S. Y. and Lee, W. C. (2008). Transcripts of enriched germ cells responding to heat shock as potential markers for porcine semen quality. Theriogenology 69(6): 758-766.
  18. Chuang, C. K., Lee, K. H., Fan, C. T. and Su, Y. S. (2007). FSH-sensitive murine sertoli cell lines immortalized by human telomerase gene hTERT express the androgen receptor in response to TNF-alpha stimulation. Biosci Rep 27(6): 403-411.
  19. Liou, M. L., Huang, S. Y., Liu, Y. C., Lin, J. H., Chuang, C. K. and Lee, W. C. (2007). Association of serum protein levels with egg productivity in Taiwan red-feathered country chickens. Anim Reprod Sci 100(1-2): 158-171.
  20. Lee, K. H., Chuang, C. K., Wang, H. W., Stone, L., Chen, C. H. and Tu, C. F. (2007). An alternative simple method for mass production of chimeric embryos by coculturing denuded embryos and embryonic stem cells in Eppendorf vials. Theriogenology 67(2): 228-237.

  21. Huang, S. Y., Lin, J. H., Chen, Y. H., Chuang, C. K., Chiu, Y. F., Chen, M. Y., Chen, H. H. and Lee, W. C. (2006). Analysis of chicken serum proteome and differential protein expression during development in single-comb White Leghorn hens. Proteomics 6(7): 2217-2224.
  22. Chan, W. E., Chuang, C. K., Yeh, S. H., Chang, M. S. and Chen, S. S. (2006). Functional characterization of heptad repeat 1 and 2 mutants of the spike protein of severe acute respiratory syndrome coronavirus. J Virol 80(7): 3225-3237.
  23. Huang, S. Y., Tam, M. F., Hsu, Y. T., Lin, J. H., Chen, H. H., Chuang, C. K., Chen, M. Y., King, Y. T. and Lee, W. C. (2005). Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis. Theriogenology 64(9): 1940-1955.
  24. Huang, S. Y., Lin, J. H., Chen, Y. H., Chuang, C. K., Lin, E. C., Huang, M. C., Sunny Sun, H. F. and Lee, W. C. (2005). A reference map and identification of porcine testis proteins using 2-DE and MS. Proteomics 5(16): 4205-4212.
1 Protocol published
Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors
Authors:  Chin-kai Chuang, Ching-Fu Tu and Chien-Hong Chen, date: 06/05/2017, view: 1459, Q&A: 0
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription ...