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Coauthors
Zhaoxia Qu University of Pittsburgh Cancer Institute, University of Pittsburgh, USA
2 protocols

Gutian Xiao University of Pittsburgh Cancer Institute, University of Pittsburgh, USA
2 protocols

Reviewer
Fan Sun
  • University of Pittsburgh
Research focus
  • Cancer biology
  • 2 Author merit
2 Protocols published
Isolation of Murine Alveolar Type II Epithelial Cells
Authors:  Fan Sun, Gutian Xiao and Zhaoxia Qu, date: 05/20/2017, view: 1278, Q&A: 0
We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar ...
Murine Bronchoalveolar Lavage
Authors:  Fan Sun, Gutian Xiao and Zhaoxia Qu, date: 05/20/2017, view: 1629, Q&A: 0
A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, ...