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Coauthors
Miho Terao Department of Systems BioMedicine, National Research Institute for Child Health and Development, Japan
1 protocol

Satoshi Hara Department of Systems BioMedicine, National Research Institute for Child Health and Development, Japan
1 protocol

Shuji Takada
Contributions
  • 1 Author merit
1 Protocol published
A Protocol for Production of Mutant Mice Using Chemically Synthesized crRNA/tracrRNA with Cas9 Nickase and FokI-dCas9
Authors:  Satoshi Hara, Miho Terao and Shuji Takada, date: 06/05/2017, view: 1230, Q&A: 0
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is the most widely used genome editing tool. A common CRISPR/Cas9 system consists of two components: a single-guide RNA (sgRNA) and ...