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Coauthors
Lei Qi Stanford Chemistry, Engineering & Medicine for Human Health (ChEM-H), Stanford University, USA, USA,
1 protocol

Xiaowo Wang Tsinghua University
1 protocol

Reviewer
Honglei Liu
  • Capital Medical University
  • 1 Author merit

Education

Ph.D, Tsinghua University, China, 2016

Current position

Lecturer, Biomedical Engineering, Capital Medical University, China

Publications

  1. Liu, H., Wei, Z., Dominguez, A., Li, Y., Wang, X. and Qi, L. S. (2015). CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation. Bioinformatics 31(22): 3676-3678.
  2. Liu, H., Li, Y. and Wang, X. (2014). OP-Synthetic: identification of optimal genetic manipulations for the overproduction of native and non-native metabolites. Quantitative Biology 2(3): 100-109.
  3. Yu, C., Liu, Y., Ma, T., Liu, K., Xu, S., Zhang, Y., Liu, H., La Russa, M., Xie, M., Ding, S. and Qi, L. S. (2015). Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16(2): 142-147.
  4. Chen, Y., Ding, Y., Yang, L., Yu, J., Liu, G., Wang, X., Zhang, S., Yu, D., Song, L., Zhang, H., Zhang, C., Huo, L., Huo, C., Wang, Y., Du, Y., Zhang, H., Zhang, P., Na, H., Xu, S., Zhu, Y., Xie, Z., He, T., Zhang, Y., Wang, G., Fan, Z., Yang, F., Liu, H., Wang, X., Zhang, X., Zhang, M. Q., Li, Y., Steinbuchel, A., Fujimoto, T., Cichello, S., Yu, J. and Liu, P. (2014). Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630. Nucleic Acids Res 42(2): 1052-1064.
1 Protocol published
Using CRISPR-ERA Webserver for sgRNA Design
Authors:  Honglei Liu, Xiaowo Wang and Lei S. Qi, date: 09/05/2017, view: 983, Q&A: 0
The CRISPR-Cas9 system is emerging as a powerful technology for gene editing (modifying the genome sequence) and gene regulation (without modifying the genome sequence). Designing sgRNAs for specific genes or regions of interest is indispensable to ...