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Coreviewers
Arsalan Daudi University of California
153 protocols

Marc-Antoine Sani University of Melbourne
8 protocols

Saswata Sankar Sarkar Stanford University
1 protocol

Reviewer
Ching Yao Yang
  • New York University
Research focus
  • Biochemistry
3 Protocols reviewed
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
Authors:  Akira Karasawa and Toshimitsu Kawate, date: 09/05/2017, view: 1097, Q&A: 0
The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
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Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Author:  Marina Verkhovskaya, date: 05/05/2017, view: 1162, Q&A: 0
The protocol for obtaining electrically sealed membrane vesicles from E. coli cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by ...
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