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Coauthors
Karl Kuchler Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Austria
5 protocols

Fabian Istel Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Austria
4 protocols

Tobias Schwarzmüller Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Austria
3 protocols
Coreviewers
Aksiniya Asenova University of California, Davis
10 protocols

Arsalan Daudi University of California
153 protocols

Christian Sailer John Innes Centre
3 protocols

Dennis Nürnberg Imperial College London
27 protocols

Reviewer
Michael Tscherner
  • Post-Doc, Harvard Medical School
Research focus
  • Molecular biology
  • Synthetic Biology
  • 4 Author merit

Education

PhD, University of Vienna, 2014

Lab information

Pamela Silver Lab
https://silver.med.harvard.edu/

4 Protocols published
Large-scale Phenotypic Profiling of Gene Deletion Mutants in Candida glabrata
Authors:  Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 3219, Q&A: 0
Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, ...
Genetic Transformation of Candida glabrata by Heat Shock
Authors:  Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 3180, Q&A: 0
Here, we report a method for the transformation of Candida glabrata using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the ...
4 Protocols reviewed
A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 351, Q&A: 0
This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
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Fluorescently Labelled Aerolysin (FLAER) Labelling of Candida albicans Cells
Authors:  Sneh Lata Singh and Sneha Sudha Komath, date: 06/05/2017, view: 1171, Q&A: 0
In this protocol we describe a nonradiolabelled labelling of GPI anchor in Candida albicans. The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be ...
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