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Coauthors
Yasuyuki Nakamura Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan
2 protocols

Jun Ishii Organization of Advanced Science and Technology, Kobe University, Japan
1 protocol

Reviewer
Yasuyuki Nakamura
  • Kobe University
Research focus
  • Microbiology
  • 1 Author merit

Education

Ph.D. in Engineering, Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan, 2014

Current position

Technology Research Association of Highly Efficient Gene Design (TRAHED) (04/2014-present)

Publications

  1. Nakamura, Y., Ishii, J. and Kondo, A. (2014). Construction of a yeast-based signaling biosensor for human angiotensin II type 1 receptor via functional coupling between Asn295-mutated receptor and Gpa1/G chimeric Galpha. Biotechnol Bioeng.
  2. Nakamura, Y., Takemoto, N., Ishii, J. and Kondo, A. (2014). Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system. Biotechnol Bioeng 111(3): 586-596.
  3. Nakamura, Y., Ishii, J. and Kondo, A. (2013). Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells. PLoS One 8(12): e82237.
  4. Nakamura, Y., Ishii, J. and Kondo, A. (2013). Rapid, Facile Detection of Heterodimer Partners for Target Human G-Protein-Coupled Receptors Using a Modified Split-Ubiquitin Membrane Yeast Two-Hybrid System. PLoS One 8(6): e66793.
1 Protocol published
Signaling Assays for Detection of Human G-protein-coupled Receptors in Yeast
Authors:  Yasuyuki Nakamura, Jun Ishii and Akihiko Kondo, date: 08/20/2014, view: 4330, Q&A: 0
G-protein-coupled receptors (GPCRs) are the largest group of cell-surface proteins and are major molecular targets for drug development. The protocol described herein is for the detection of human GPCR signaling in the yeast Saccharomyces cerevisiae ...