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Coauthors
George Diallinas Faculty of Biology, University of Athens, Greece
1 protocol
Coreviewers
Belen Sanz Complutense University
6 protocols

Emily Cope Northern Arizona University
6 protocols

Fanglian He University of Pennsylvania
205 protocols

Jose Thekkiniath Yale University
1 protocol

Reviewer
Emilia Krypotou
  • Research scientist, University of Edinburgh
Research focus
  • Microbiology
  • Molecular Microbiology, Bacterial pathogens, Fungal transporters
  • 1 Author merit

Education

PhD, National and Kapodistrian University of Athens

Lab information

Jose A. Vazquez-Boland lab

Publications

1. Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. Modeling, substrate docking, and mutational analysis identify residues essential for the function and specificity of a eukaryotic purine-cytosine NCS1 transporter. J Biol Chem. 2012, 287: 36792-803.
2. Krypotou E, Diallinas G. Transport assays in filamentous fungi: kinetic characterization of the UapC purine transporter of Aspergillus nidulans. Fungal Genet Biol. 2014, 63: 1-8. doi: 10.1016/j.fgb.2013.12.004.
3. Krypotou E, Lambrinidis G, Evangelidis T, Mikros E, Diallinas G. Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA. Mol Microbiol. 2014, 93: 129-45. doi: 10.1111/mmi.12646.
4. Krypotou E, Scazzocchio C, Diallinas G. Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genet Biol. 2015, 75: 56-63. doi: 10.1016/j.fgb.2015.01.009.
5. Krypotou E., Evangelidis T., Bobonis J., Pittis A.A., Gabaldón T., Scazzocchio C., Mikros E., Diallinas G. Origin, diversification and substrate specificity in the family of NCS1/FUR transporters. Mol Microbiol. 2015, 96: 927-50. doi: 10.1111/mmi.12982.
6. Lougiakis N, Gavriil ES, Kairis M, Sioupouli G, Lambrinidis G, Benaki D, Krypotou E, Mikros E, Marakos P, Pouli N, Diallinas G. Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter. Bioorg Med Chem. 2016, 24: 5941-52.

1 Protocol published
Transport Assays in Aspergillus nidulans
Authors:  Emilia Krypotou and George Diallinas, date: 11/20/2013, view: 3386, Q&A: 0
Transport assays allow the direct kinetic analysis of a specific transporter by measuring apparent Km and Vmax values, and permit the characterization of substrate specificity profiles through competition ...
6 Protocols reviewed
Highly Accurate Real-time Measurement of Rapid Hydrogen-peroxide Dynamics in Fungi
Authors:  Michael Mentges and Jörg Bormann, date: 12/20/2016, view: 2204, Q&A: 0
Reactive oxygen species (ROS) are unavoidable by-products of aerobic metabolism. Despite beneficial aspects as a signaling molecule, ROS are principally recognized as harmful agents that act on nucleic acids, proteins and lipids. Reactive oxygen ...
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A Highly Efficient Method for Measuring Oxygen Consumption Rate in Fusarium graminearum
Authors:  Daniel Gebhard, Jakob Bönnighausen, Jörg Bergemann, Wilhelm Schäfer and Jörg Bormann, date: 08/05/2016, view: 2497, Q&A: 0
The filamentous ascomycete Fusarium graminearum is the causal agent of Fusarium head blight, a devastating disease of cereals with a worldwide distribution. Fusarium graminearum infections result in a quantitative yield ...
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