Elias Haddad Vaccine and Gene Therapy Institute Florida, Port St Lucie, USA
1 protocol

Rafael A. Cubas
  • 1 Author merit
  • 3 Reviewer merit


Ph.D. in Molecular Virology and Microbiology, Department of Molecular Virology & Microbiology, Baylor College of Medicine, 2010

Current Position

Post-doctoral researcher at the Vaccine and Gene Therapy Institute of Florida, Port St. Lucie, Florida, United States


  1. Locci, M., Havenar-Daughton, C., Landais, E., Wu, J., Kroenke, M. A., Arlehamn, C. L., Su, L. F., Cubas, R., Davis, M. M., Sette, A., Haddad, E. K., Poignard, P. and Crotty, S. (2013). Human circulating PD-(+)1CXCR3(-)CXCR5(+) memory Tfh cells are highly functional and correlate with broadly neutralizing HIV antibody responses. Immunity 39(4): 758-769.
  2. Zhang, S., Yong, L. K., Li, D., Cubas, R., Chen, C. and Yao, Q. (2013). Mesothelin virus-like particle immunization controls pancreatic cancer growth through CD8+ T cell induction and reduction in the frequency of CD4+ foxp3+ ICOS- regulatory T cells. PLoS One 8(7): e68303.
  3. Cubas, R. A., Mudd, J. C., Savoye, A. L., Perreau, M., van Grevenynghe, J., Metcalf, T., Connick, E., Meditz, A., Freeman, G. J., Abesada-Terk, G., Jr., Jacobson, J. M., Brooks, A. D., Crotty, S., Estes, J. D., Pantaleo, G., Lederman, M. M. and Haddad, E. K. (2013). Inadequate T follicular cell help impairs B cell immunity during HIV infection. Nat Med 19(4): 494-499.
  4. Mudd, J. C., Murphy, P., Manion, M., Debernardo, R., Hardacre, J., Ammori, J., Hardy, G. A., Harding, C. V., Mahabaleshwar, G. H., Jain, M. K., Jacobson, J. M., Brooks, A. D., Lewis, S., Schacker, T. W., Anderson, J., Haddad, E. K., Cubas, R. A., Rodriguez, B., Sieg, S. F. and Lederman, M. M. (2013). Impaired T-cell responses to sphingosine-1-phosphate in HIV-1 infected lymph nodes. Blood 121(15): 2914-2922.
  5. Perreau, M., Savoye, A. L., De Crignis, E., Corpataux, J. M., Cubas, R., Haddad, E. K., De Leval, L., Graziosi, C. and Pantaleo, G. (2013). Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production. J Exp Med 210(1): 143-156.
  6. van Grevenynghe, J., Cubas, R. A., DaFonseca, S., Metcalf, T., Tremblay, C. L., Trautmann, L., Sekaly, R. P., Schatzle, J. and Haddad, E. K. (2012). Foxo3a: an integrator of immune dysfunction during HIV infection. Cytokine Growth Factor Rev 23(4-5): 215-221.
  7. van Grevenynghe, J., Cubas, R. A., Noto, A., DaFonseca, S., He, Z., Peretz, Y., Filali-Mouhim, A., Dupuy, F. P., Procopio, F. A., Chomont, N., Balderas, R. S., Said, E. A., Boulassel, M. R., Tremblay, C. L., Routy, J. P., Sekaly, R. P. and Haddad, E. K. (2011). Loss of memory B cells during chronic HIV infection is driven by Foxo3a- and TRAIL-mediated apoptosis. J Clin Invest 121(10): 3877-3888.
  8. Cubas, R., Zhang, S., Li, M., Chen, C. and Yao, Q. (2011). Chimeric Trop2 virus-like particles: a potential immunotherapeutic approach against pancreatic cancer. J Immunother 34(3): 251-263.
  9. Cubas, R., Zhang, S., Li, M., Chen, C. and Yao, Q. (2010). Trop2 expression contributes to tumor pathogenesis by activating the ERK MAPK pathway. Mol Cancer 9: 253.
  10. Trerotola, M., Guerra, E. and Alberti, S. (2010). Letter to the editor: efficacy and safety of anti-Trop antibodies, R. Cubas, M. Li, C. Chen and Q. Yao, Biochim Biophys Acta 1796 (2009) 309-1. Biochim Biophys Acta 1805(2): 119-120; author reply 121-112.
  11. Cubas, R., Li, M., Chen, C. and Yao, Q. (2009). Trop2: a possible therapeutic target for late stage epithelial carcinomas. Biochim Biophys Acta 1796(2): 309-314.
  12. Cubas, R., Zhang, S., Kwon, S., Sevick-Muraca, E. M., Li, M., Chen, C. and Yao, Q. (2009). Virus-like particle (VLP) lymphatic trafficking and immune response generation after immunization by different routes. J Immunother 32(2): 118-128.
  13. Zhang, S., Cubas, R., Li, M., Chen, C. and Yao, Q. (2009). Virus-like particle vaccine activates conventional B2 cells and promotes B cell differentiation to IgG2a producing plasma cells. Mol Immunol 46(10): 1988-2001.
  14. Jamaluddin, M. S., Wang, X., Wang, H., Rafael, C., Yao, Q. and Chen, C. (2009). Eotaxin increases monolayer permeability of human coronary artery endothelial cells. Arteriosclerosis, thrombosis, and vascular biology 29(12): 2146-2152.
  15. Cubas, R., Li, M., Chen, C. and Yao, Q. (2007). Colorectal cancer: new advances in immunotherapy. Cancer Biol Ther 6(1): 11-17.
1 Protocol published
T Follicular Helper Cell Coculture Assay
Authors:  Rafael A. Cubas and Elias K. Haddad, date: 01/05/2014, view: 5909, Q&A: 0
T follicular helper (Tfh) cells constitute a distinct subset of CD4+ T cells specialized in providing help to B cells in germinal centers. Phenotypically, Tfh cells are characterized by their high expression of the chemokine receptor ...