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Coauthors
Justo Castaño Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols

Raúl Luque Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols
Coreviewers
Kae-Jiun Chang University of California, San Francisco
8 protocols

Oneil Bhalala System Genomics Lab, Baker Institute
29 protocols

Renate Weizbauer Carnegie Insitution for Science, Stanford, CA
31 protocols

Shai Berlin The Ruth and Bruce Rappaport Faculty of Medicine, Technion- Israel Institute of Technology (+972)-4-829-5346 1 Efron st. Bat Galim Haifa, 3525433, Israel
5 protocols

Manuel D Gahete
  • Post-Doc, IMIBIC - Maimonides Institute for Biomedical Research of Córdoba
Research focus
  • Cancer biology
Contributions
  • 2 Author merit
  • 6 Reviewer merit

Education

PhD, University of Cordoba

2 Protocols published
Measurement of Free Cytosolic Calcium Concentration ([Ca2+]i) in Single CHO-K1 Cells
Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 5686, Q&A: 0
This is a protocol to analyze the functional response of single CHO-K1 cells to a given treatment in terms of changes in free cytosolic calcium concentration ([Ca2+]i). This is possible by using the Ca2+ indicator dye Fura-2 AM, a polyamino ...
GPCRs Interaction Measurement by Fluorescence Resonance Energy Transfer (FRET)
Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 4264, Q&A: 0
This is a protocol to determine the physical interaction of a G-protein coupled receptor (GPCR) with itself (homodimerization) or with other GPCR (heterodimerazation) using fluorescence resonance energy transfer (FRET). FRET is a distance-dependent ...
6 Protocols reviewed
Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices
Authors:  Jillian M. Daniel, Jim Deuchars and Susan A. Deuchars, date: 10/05/2016, view: 1693, Q&A: 0
In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) ...
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Measurement of Inositol Triphosphate Levels from Rat Hippocampal Slices
Authors:  Nino Tabatadze and Catherine Woolley, date: 04/05/2016, view: 1868, Q&A: 0
Inositol triphosphate (IP3) is an important second messenger that participates in signal transduction pathways in diverse cell types including hippocampal neurons. Stimulation of phospholipase C in response to various stimuli (hormones, ...
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