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Coauthors
Gyorgy Szabadkai Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
1 protocol

Tunde Berecz Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
1 protocol

Tom Blacker Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
1 protocol

Michael R. Duchen
  • Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, United Kingdom
Contributions
  • 1 Author merit

Education

Ph.D in Physiology, University College of London, 1984

Current Position

Professor of Physiology, University College London
Chair of Basic Life Sciences Domain, University College London

Publications

  1. Chisholm, K. I., Ida, K. K., Davies, A. L., Tachtsidis, I., Papkovsky, D. B., Dyson, A., Singer, M., Duchen, M. R. and Smith, K. J. (2016). Hypothermia protects brain mitochondrial function from hypoxemia in a murine model of sepsis. J Cereb Blood Flow Metab 36(11): 1955-1964.
  2. Guedes-Dias, P., Pinho, B. R., Soares, T. R., de Proenca, J., Duchen, M. R. and Oliveira, J. M. (2016). Mitochondrial dynamics and quality control in Huntington's disease. Neurobiol Dis 90: 51-57.
  3. Chisholm, K. I., Ida, K. K., Davies, A. L., Papkovsky, D. B., Singer, M., Dyson, A., Tachtsidis, I., Duchen, M. R. and Smith, K. J. (2016). In Vivo Imaging of Flavoprotein Fluorescence During Hypoxia Reveals the Importance of Direct Arterial Oxygen Supply to Cerebral Cortex Tissue. Adv Exp Med Biol 876: 233-239.
  4. Hawkins, K. E., Joy, S., Delhove, J. M., Kotiadis, V. N., Fernandez, E., Fitzpatrick, L. M., Whiteford, J. R., King, P. J., Bolanos, J. P., Duchen, M. R., Waddington, S. N. and McKay, T. R. (2016). NRF2 Orchestrates the Metabolic Shift during Induced Pluripotent Stem Cell Reprogramming. Cell Rep 14(8): 1883-1891.
  5. Bhosale, G., Sharpe, J. A., Sundier, S. Y. and Duchen, M. R. (2015). Calcium signaling as a mediator of cell energy demand and a trigger to cell death. Ann N Y Acad Sci 1350: 107-116.
  6. Davidson, S. M., Foote, K., Kunuthur, S., Gosain, R., Tan, N., Tyser, R., Zhao, Y. J., Graeff, R., Ganesan, A., Duchen, M. R., Patel, S. and Yellon, D. M. (2015). Inhibition of NAADP signalling on reperfusion protects the heart by preventing lethal calcium oscillations via two-pore channel 1 and opening of the mitochondrial permeability transition pore. Cardiovasc Res 108(3): 357-366.
  7. Guedes-Dias, P., de Proenca, J., Soares, T. R., Leitao-Rocha, A., Pinho, B. R., Duchen, M. R. and Oliveira, J. M. (2015). HDAC6 inhibition induces mitochondrial fusion, autophagic flux and reduces diffuse mutant huntingtin in striatal neurons. Biochim Biophys Acta 1852(11): 2484-2493.
  8. Ikaga, R., Namekata, I., Kotiadis, V. N., Ogawa, H., Duchen, M. R., Tanaka, H., Iida-Tanaka, N. (2015). Knockdown of aquaporin-8 induces mitochondrial dysfunction in 3T3-L1 cells. Biochemistry and Biophysics Reports 4: 187–195.
  9. Nickel, A. G., von Hardenberg, A., Hohl, M., Loffler, J. R., Kohlhaas, M., Becker, J., Reil, J. C., Kazakov, A., Bonnekoh, J., Stadelmaier, M., Puhl, S. L., Wagner, M., Bogeski, I., Cortassa, S., Kappl, R., Pasieka, B., Lafontaine, M., Lancaster, C. R., Blacker, T. S., Hall, A. R., Duchen, M. R., Kastner, L., Lipp, P., Zeller, T., Muller, C., Knopp, A., Laufs, U., Bohm, M., Hoth, M. and Maack, C. (2015). Reversal of Mitochondrial Transhydrogenase Causes Oxidative Stress in Heart Failure. Cell Metab 22(3): 472-484.
  10. Schwarzlander, M., Wagner, S., Ermakova, Y. G., Belousov, V. V., Radi, R., Beckman, J. S., Buettner, G. R., Demaurex, N., Duchen, M. R., Forman, H. J., Fricker, M. D., Gems, D., Halestrap, A. P., Halliwell, B., Jakob, U., Johnston, I. G., Jones, N. S., Logan, D. C., Morgan, B., Muller, F. L., Nicholls, D. G., Remington, S. J., Schumacker, P. T., Winterbourn, C. C., Sweetlove, L. J., Meyer, A. J., Dick, T. P. and Murphy, M. P. (2014). The 'mitoflash' probe cpYFP does not respond to superoxide. Nature 514(7523): E12-14.
  11. Chouchani, E. T., Pell, V. R., Gaude, E., Aksentijevic, D., Sundier, S. Y., Robb, E. L., Logan, A., Nadtochiy, S. M., Ord, E. N., Smith, A. C., Eyassu, F., Shirley, R., Hu, C. H., Dare, A. J., James, A. M., Rogatti, S., Hartley, R. C., Eaton, S., Costa, A. S., Brookes, P. S., Davidson, S. M., Duchen, M. R., Saeb-Parsy, K., Shattock, M. J., Robinson, A. J., Work, L. M., Frezza, C., Krieg, T. and Murphy, M. P. (2014). Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature 515(7527): 431-435.
  12. Blacker, T. S., Mann, Z. F., Gale, J. E., Ziegler, M., Bain, A. J., Szabadkai, G. and Duchen, M. R. (2014). Separating NADH and NADPH fluorescence in live cells and tissues using FLIM. Nat Commun 5: 3936.
  13. Logan, C. V., Szabadkai, G., Sharpe, J. A., Parry, D. A., Torelli, S., Childs, A. M., Kriek, M., Phadke, R., Johnson, C. A., Roberts, N. Y., Bonthron, D. T., Pysden, K. A., Whyte, T., Munteanu, I., Foley, A. R., Wheway, G., Szymanska, K., Natarajan, S., Abdelhamed, Z. A., Morgan, J. E., Roper, H., Santen, G. W., Niks, E. H., van der Pol, W. L., Lindhout, D., Raffaello, A., De Stefani, D., den Dunnen, J. T., Sun, Y., Ginjaar, I., Sewry, C. A., Hurles, M., Rizzuto, R., Consortium, U. K., Duchen, M. R., Muntoni, F. and Sheridan, E. (2014). Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling. Nat Genet 46(2): 188-193.
  14. Sajic, M., Mastrolia, V., Lee, C. Y., Trigo, D., Sadeghian, M., Mosley, A. J., Gregson, N. A., Duchen, M. R. and Smith, K. J. (2013). Impulse conduction increases mitochondrial transport in adult mammalian peripheral nerves in vivo. PLoS Biol 11(12): e1001754.
  15. Osellame, L. D., Rahim, A. A., Hargreaves, I. P., Gegg, M. E., Richard-Londt, A., Brandner, S., Waddington, S. N., Schapira, A. H. V. and Duchen, M. R. (2013). Accumulation of damaged mitochondria in Gaucher Disease is due to defective cellular degradation machinery. Cell Metabolism 17(6):941-953.
  16. Faccenda, D., Tan, C. H., Seraphim, A., Duchen, M. R. and Campanella, M. (2013). IF1 limits the apoptotic-signalling cascade by preventing mitochondrial remodelling. Cell Death Differ 20(5): 686-697.
  17. Traba, J., Szabadkai, G., del Arco, A., Duchen, M. R. and Satrústegui, J. (2012). SCaMC-1 promotes cancer cell survival by desensitizing mitochondrial permeability transition via ATP/ADP-mediated matrix Ca2+ buffering. Cell Death and Differentiation 19(4): 650-660.
  18. Davidson, S. M., Yellon, D. M., Murphy, M. P. and Duchen, M. R. (2012). Slow calcium waves and redox changes precede mitochondrial permeability transition pore opening in the intact heart during hypoxia and reoxygenation. Cardiovasc Res 93(3): 445-453.
  19. Girard, M., Lariviere, R., Parfitt, D. A., Deane, E. C., Gaudet, R., Nossova, N., Blondeau, F., Prenosil, G., Vermeulen, E. G., Duchen, M. R., Richter, A., Shoubridge, E. A., Gehring, K., McKinney, R. A., Brais, B., Chapple, J. P. and McPherson, P. S. (2012). Mitochondrial dysfunction and Purkinje cell loss in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). Proc Natl Acad Sci U S A 109(5): 1661-1666.
  20. Abramov, A. Y., Ionov, M., Pavlov, E. and Duchen, M. R. (2011). Membrane cholesterol content plays a key role in the neurotoxicity of beta-amyloid: implications for Alzheimer's disease. Aging Cell 10(4): 595-603.
  21. Abeti, R., Abramov, A. Y. and Duchen, M. R. (2011). Beta-amyloid activates PARP causing astrocytic metabolic failure and neuronal death. Brain 134(Pt 6): 1658-1672.
  22. Galkin, A., Abramov, A. Y., Frakich, N., Duchen, M. R. and Moncada, S. (2010). Lack of oxygen deactivates mitochondrial complex I: implications for ischemic injury? J Biol Chem 284(52):36055-36061.
  23. Abramov, A. Y., Smulders-Srinivasan, T. K., Kirby, D. M., Acin-Perez, R., Enriquez, J. A., Lightowlers, R. N., Duchen, M. R. and Turnbull, D. M. (2010). Mechanism of neurodegeneration of neurons with mitochondrial DNA mutations. Brain 133(Pt 3): 797-807.
  24. Hall, A. M., Unwin, R. J., Parker, N. and Duchen, M. R. (2009). Multiphoton imaging reveals differences in mitochondrial function between nephron segments. J Am Soc Nephrol 20(6): 1293-1302.
  25. Milton, R. H., Abeti, R., Averaimo, S., DeBiasi, S., Vitellaro, L., Jiang, L., Curmi, P. M., Breit, S. N., Duchen, M. R. and Mazzanti, M. (2008). CLIC1 function is required for beta-amyloid-induced generation of reactive oxygen species by microglia. J Neurosci 28(45): 11488-11499.
  26. Campanella, M., Casswell, E., Chong, S., Farah, Z., Wieckowski, M. R., Abramov, A. Y., Tinker, A. and Duchen, M. R. (2008). Regulation of mitochondrial structure and function by the F1Fo-ATPase inhibitor protein, IF1. Cell Metab 8(1): 13-25.
1 Protocol published
Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime
Authors:  Thomas S. Blacker, Tunde Berecz, Michael R. Duchen and Gyorgy Szabadkai, date: 01/20/2017, view: 2085, Q&A: 0
NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role in cellular antioxidant defence. In live cells and tissues, the intensity of their ...