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Coauthors
Ilona Kammerl Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Germany
1 protocol

Silke Meiners Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Germany
1 protocol

Hermen Overkleeft Department of Bio-organic Synthesis, Leiden University, The Netherlands
1 protocol

Bogdan Florea Department of Bio-organic Synthesis, Leiden University, The Netherlands
1 protocol

Oliver Vosyka
  • Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University and Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Germany
Contributions
  • 1 Author merit
1 Protocol published
Activity-based Pull-down of Proteolytic Standard and Immunoproteasome Subunits
Activity-based probes (ABP) are small organic molecules that irreversibly bind to the active center of a specific enzyme family and may be coupled to a fluorophore or an affinity tag (Li et al., 2013). Here, we describe a method to ...