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Coauthors
Hiroyuki Konishi Department of Biochemistry, Aichi Medical University School of Medicine, Japan
1 protocol

Yoshitaka Hosokawa Department of Biochemistry, Aichi Medical University School of Medicine, Japan
1 protocol

Shinobu Tsuzuki Department of Biochemistry, Aichi Medical University School of Medicine, Japan
1 protocol

Md Wahiduzzaman Department of Biochemistry, Aichi Medical University School of Medicine, Japan
1 protocol

Sivasundaram Karnan
  • Department of Biochemistry, Aichi Medical University School of Medicine, Japan
  • 1 Author merit

Education

Ph.D in Medical Science, Nagoya City University Graduate School of Medicine

Current Position

Lecturer, Department of Biochemistry, Aichi Medical University School of Medicine, Japan

Publications (since 2010)

  1. Ito, K., Ota, A., Ono, T., Nakaoka, T., Wahiduzzaman, M., Karnan, S., Konishi, H., Furuhashi, A., Hayashi, T., Yamada, Y., Hosokawa, Y. and Kazaoka, Y. (2016). Inhibition of Nox1 induces apoptosis by attenuating the AKT signaling pathway in oral squamous cell carcinoma cell lines. Oncol Rep 36(5): 2991-2998.
  2. Karnan, S., Ota, A., Konishi, Y., Wahiduzzaman, M., Hosokawa, Y. and Konishi, H. (2016). Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide. Nucleic Acids Res 44(6): e54.
  3. Mizuno, S., Hanamura, I., Ota, A., Karnan, S., Narita, T., Ri, M., Mizutani, M., Goto, M., Gotou, M., Tsunekawa, N., Shikami, M., Iida, S., Hosokawa, Y., Miwa, H., Ueda, R., Nitta, M. and Takami, A. (2015). Overexpression of salivary-type amylase reduces the sensitivity to bortezomib in multiple myeloma cells. Int J Hematol 102(5): 569-578.
  4. Tanaka, M., Miura, Y., Numanami, H., Karnan, S., Ota, A., Konishi, H., Hosokawa, Y. and Hanyuda, M. (2015). Inhibition of NADPH oxidase 4 induces apoptosis in malignant mesothelioma: Role of reactive oxygen species. Oncol Rep 34(4): 1726-1732.
  5. Ono, T., Ota, A., Ito, K., Nakaoka, T., Karnan, S., Konishi, H., Furuhashi, A., Hayashi, T., Yamada, Y., Hosokawa, Y. and Kazaoka, Y. (2015). Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines. Oral Dis 21(4): 501-511.
  6. Asai, A., Karnan, S., Ota, A., Takahashi, M., Damdindorj, L., Konishi, Y., Hossain, E., Konishi, H., Nagata, A., Yokoo, K. and Hosokawa, Y. (2015). High-resolution 400K oligonucleotide array comparative genomic hybridization analysis of neurofibromatosis type 1-associated cutaneous neurofibromas. Gene 558(2): 220-226.
  7. Hossain, E., Ota, A., Karnan, S., Takahashi, M., Mannan, S. B., Konishi, H. and Hosokawa, Y. (2015). Lipopolysaccharide augments the uptake of oxidized LDL by up-regulating lectin-like oxidized LDL receptor-1 in macrophages. Mol Cell Biochem 400(1-2): 29-40.
  8. Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y. and Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated viral vectors. PLoS One 9(8): e106472.
  9. Guo, Y., Takeuchi, I., Karnan, S., Miyata, T., Ohshima, K. and Seto, M. (2014). Array-comparative genomic hybridization profiling of immunohistochemical subgroups of diffuse large B-cell lymphoma shows distinct genomic alterations. Cancer Sci 105(4): 481-489.
  10. Suguro, M., Yoshida, N., Umino, A., Kato, H., Tagawa, H., Nakagawa, M., Fukuhara, N., Karnan, S., Takeuchi, I., Hocking, T. D., Arita, K., Karube, K., Tsuzuki, S., Nakamura, S., Kinoshita, T. and Seto, M. (2014). Clonal heterogeneity of lymphoid malignancies correlates with poor prognosis. Cancer Sci 105(7): 897-904.
  11. Nakaoka, T., Ota, A., Ono, T., Karnan, S., Konishi, H., Furuhashi, A., Ohmura, Y., Yamada, Y., Hosokawa, Y. and Kazaoka, Y. (2014). Combined arsenic trioxide-cisplatin treatment enhances apoptosis in oral squamous cell carcinoma cells. Cell Oncol (Dordr) 37(2): 119-129.
  12. Hossain, E., Ota, A., Karnan, S., Damdindorj, L., Takahashi, M., Konishi, Y., Konishi, H. and Hosokawa, Y. (2013). Arsenic augments the uptake of oxidized LDL by upregulating the expression of lectin-like oxidized LDL receptor in mouse aortic endothelial cells. Toxicol Appl Pharmacol 273(3): 651-658.
  13. Wang, G. M., Wong, H. Y., Konishi, H., Blair, B. G., Abukhdeir, A. M., Gustin, J. P., Rosen, D. M., Denmeade, S. R., Rasheed, Z., Matsui, W., Garay, J. P., Mohseni, M., Higgins, M. J., Cidado, J., Jelovac, D., Croessmann, S., Cochran, R. L., Karnan, S., Konishi, Y., Ota, A., Hosokawa, Y., Argani, P., Lauring, J. and Park, B. H. (2013). Single copies of mutant KRAS and mutant PIK3CA cooperate in immortalized human epithelial cells to induce tumor formation. Cancer Res 73(11): 3248-3261.
  14. Takahashi, M., Ota, A., Karnan, S., Hossain, E., Konishi, Y., Damdindorj, L., Konishi, H., Yokochi, T., Nitta, M. and Hosokawa, Y. (2013). Arsenic trioxide prevents nitric oxide production in lipopolysaccharide -stimulated RAW 264.7 by inhibiting a TRIF-dependent pathway. Cancer Sci 104(2): 165-170.
  15. Hossain, E., Ota, A., Takahashi, M., Karnan, S., Damdindorj, L., Konishi, Y., Konishi, H. and Hosokawa, Y. (2013). Arsenic upregulates the expression of angiotensin II Type I receptor in mouse aortic endothelial cells. Toxicol Lett 220(1): 70-75.
  16. Karnan, S., Konishi, Y., Ota, A., Takahashi, M., Damdindorj, L., Hosokawa, Y. and Konishi, H. (2012). Simple monitoring of gene targeting efficiency in human somatic cell lines using the PIGA gene. PLoS One 7(10): e47389.
  17. Konishi, Y., Karnan, S., Takahashi, M., Ota, A., Damdindorj, L., Hosokawa, Y. and Konishi, H. (2012). A system for the measurement of gene targeting efficiency in human cell lines using an antibiotic resistance-GFP fusion gene. Biotechniques 53(3): 141-152.
  18. Damdindorj, L., Karnan, S., Ota, A., Takahashi, M., Konishi, Y., Hossain, E., Hosokawa, Y. and Konishi, H. (2012). Assessment of the long-term transcriptional activity of a 550-bp-long human beta-actin promoter region. Plasmid 68(3): 195-200.
  19. Konishi, H., Mohseni, M., Tamaki, A., Garay, J. P., Croessmann, S., Karnan, S., Ota, A., Wong, H. Y., Konishi, Y., Karakas, B., Tahir, K., Abukhdeir, A. M., Gustin, J. P., Cidado, J., Wang, G. M., Cosgrove, D., Cochran, R., Jelovac, D., Higgins, M. J., Arena, S., Hawkins, L., Lauring, J., Gross, A. L., Heaphy, C. M., Hosokawa, Y., Gabrielson, E., Meeker, A. K., Visvanathan, K., Argani, P., Bachman, K. E. and Park, B. H. (2011). Mutation of a single allele of the cancer susceptibility gene BRCA1 leads to genomic instability in human breast epithelial cells. Proc Natl Acad Sci U S A 108(43): 17773-17778.
  20. Sung, C. O., Kim, S. C., Karnan, S., Karube, K., Shin, H. J., Nam, D. H., Suh, Y. L., Kim, S. H., Kim, J. Y., Kim, S. J., Kim, W. S., Seto, M. and Ko, Y. H. (2011). Genomic profiling combined with gene expression profiling in primary central nervous system lymphoma. Blood 117(4): 1291-1300.
  21. Kagami, Y., Karnan, S., Nakagawa, A., Oshiro, K., Kato, H., Koike, K., Morishima, Y. and Seto, M. (2011). Establishment of an adult T-cell leukemia cell line (HU-ATTAK) dependent for proliferation on human umbilical cord vein endothelial cells. The Open Leukemia Journal 4: 1-8。
  22. Karnan, S., Mohseni, M., Konishi, Y., Tamaki, A., Hosokawa, Y., Park, B. H. and Konishi, H. (2010). Controversial BRCA1 allelotypes in commonly used breast cancer cell lines. Breast Cancer Res Treat 119(1): 249-251.
  23. Ko, Y. H., Karnan, S., Kim, K. M., Park, C. K., Kang, E. S., Kim, Y. H., Kang, W. K., Kim, S. J., Kim, W. S., Lee, W. Y., Chun, H. K. and Seto, M. (2010). Enteropathy-associated T-cell lymphoma--a clinicopathologic and array comparative genomic hybridization study. Hum Pathol 41(9): 1231-1237.
  24. Kato, H., Kagami, Y., Nakagawa, M., Karnan, S., Yatabe, Y., Nakamura, S., Morishima, Y. and Seto, M. (2010). FIL-4/CD40L co-stimulation induces long-term proliferation for CD10-positive germinal center B cell-like diffuse large B-cell lymphomas. The Open Leukemia Journal 3: 60-68.






1 Protocol published
Efficient AAV-mediated Gene Targeting Using 2A-based Promoter-trap System
Adeno-associated virus (AAV)-based targeting vectors have 1-4-log higher gene targeting efficiencies compared with plasmid-based targeting vectors. The efficiency of AAV-mediated gene targeting is further increased by introducing a promoter-trap ...