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Coauthors
Corinne Mercier Université Grenoble Alpes, France
1 protocol

Jean Gagnon Labroatoire Adaptation et Pathogénie des Microorganismes (LAPM), France
2 protocols

Marie-France Cesbron-Delauw Université Grenoble Alpes, France
2 protocols

Nicolas Blanchard Centre de Physiopathologie de Toulouse-Purpan (CPTP), France
1 protocol

Amina Bittame
  • Labroatoire Adaptation et Pathogénie des Microorganismes (LAPM), France
Contributions
  • 1 Author merit

Education

Ph. D in Virology, Microbiology, Immunology, Université de Grenoble, France, 2011

Publications

  1. Lopez, J., Bittame, A., Massera, C., Vasseur, V., Effantin, G., Valat, A., Buaillon, C., Allart, S., Fox, B. A., Rommereim, L. M., Bzik, D. J., Schoehn, G., Weissenhorn, W., Dubremetz, J. F., Gagnon, J., Mercier, C., Cesbron-Delauw, M. F. and Blanchard, N. (2015). Intravacuolar Membranes Regulate CD8 T Cell Recognition of Membrane-Bound Toxoplasma gondii Protective Antigen. Cell Rep 13(10): 2273-2286.

  2. Bittame, A., Effantin, G., Petre, G., Ruffiot, P., Travier, L., Schoehn, G., Weissenhorn, W., Cesbron-Delauw, M. F., Gagnon, J. and Mercier, C. (2015). Toxoplasma gondii: biochemical and biophysical characterization of recombinant soluble dense granule proteins GRA2 and GRA6. Biochem Biophys Res Commun 459(1): 107-112.

  3. Koethe, M., Pott, S., Ludewig, M., Bangoura, B., Zoller, B., Daugschies, A., Tenter, A. M., Spekker, K., Bittame, A., Mercier, C., Fehlhaber, K. and Straubinger, R. K. (2011). Prevalence of specific IgG-antibodies against Toxoplasma gondii in domestic turkeys determined by kinetic ELISA based on recombinant GRA7 and GRA8. Vet Parasitol 180(3-4): 179-190.

  4. Gendrin, C., Bittame, A., Mercier, C. and Cesbron-Delauw, M. F. (2010). Post-translational membrane sorting of the Toxoplasma gondii GRA6 protein into the parasite-containing vacuole is driven by its N-terminal domain. Int J Parasitol 40(11): 1325-1334.

  5. Michelin, A., Bittame, A., Bordat, Y., Travier, L., Mercier, C., Dubremetz, J. F. and Lebrun, M. (2009). GRA12, a Toxoplasma dense granule protein associated with the intravacuolar membranous nanotubular network. Int J Parasitol 39(3): 299-306.

  6. Mercier, C., Travier, L., Bittame, A., Gendrin, C. and Cesbron-Delauw, M. F. (2010). The dense granule proteins of Toxoplasma gondii. In: De Bruyn, O. and Peeters, S. (eds). Parasitology Research Trends. Nova Science Publishers, pp 1-31. ISBN: 978-1-60741-436-0.

1 Protocol published
Lipid Extraction from HeLa Cells, Quantification of Lipids, Formation of Large Unilamellar Vesicles (LUVs) by Extrusion and in vitro Protein-lipid Binding Assays, Analysis of the Incubation Product by Transmission Electron Microscopy (TEM) and by Flotation across a Discontinuous Sucrose Gradient
Dissecting the interactions established between proteins and membranes in a given type of cells is not an easy task. Using a cell-free system of large unilamellar vesicles (LUVs) to analyze these interactions may help decipher these interactions and ...