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Coauthors
Carlos Duarte CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
2 protocols

Simone Frisari CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
1 protocol

Bárbara Correia CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
1 protocol

Rui Costa CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
1 protocol

Margarida Caldeira
  • CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
  • 2 Author merit

Education

Ph.D. in Cell Biology, Center for Neurosciences and Cell Biology of Coimbra and Ph.D. Programme in Biomedicine and Experimental Biology, University of Coimbra

Current position

Post Doctoral Fellow, Center for Neuroscience and Cell Biology of Coimbra- Microscopy Unit

Publications

  1. Salazar, I. L., Caldeira, M. V., Curcio, M. and Duarte, C. B. (2016). The Role of Proteases in Hippocampal Synaptic Plasticity: Putting Together Small Pieces of a Complex Puzzle. Neurochem Res 41(1-2): 156-182.
  2. Caldeira, M. V., Salazar, I. L., Curcio, M., Canzoniero, L. M. and Duarte, C. B. (2014). Role of the ubiquitin-proteasome system in brain ischemia: friend or foe? Prog Neurobiol 112: 50-69.
  3. Caldeira, M. V., Curcio, M., Leal, G., Salazar, I. L., Mele, M., Santos, A. R., Melo, C. V., Pereira, P., Canzoniero, L. M. and Duarte, C. B. (2013). Excitotoxic stimulation downregulates the ubiquitin-proteasome system through activation of NMDA receptors in cultured hippocampal neurons. Biochim Biophys Acta 1832(1): 263-274.
  4. Baptista, M. S., Melo, C. V., Armelao, M., Herrmann, D., Pimentel, D. O., Leal, G., Caldeira, M. V., Bahr, B. A., Bengtson, M., Almeida, R. D. and Duarte, C. B. (2010). Role of the proteasome in excitotoxicity-induced cleavage of glutamic acid decarboxylase in cultured hippocampal neurons. PLoS One 5(4): e10139.
  5. Santos, S. D., Carvalho, A. L., Caldeira, M. V. and Duarte, C. B. (2009). Regulation of AMPA receptors and synaptic plasticity. Neuroscience 158(1): 105-125.
  6. Carvalho, A. L., Caldeira, M. V., Gomes, A. R., Carvalho, A. P. and Duarte, C. B. (2008). Regulation of AMPA receptors by metabotropic receptors and receptor tyrosine kinases: mechanisms and physiological roles. In: Hamon, M. and Vizi, E. S. (eds). Handbook of Neurochemistry & Molecular Neurobiology, volume 2 Neurotransmitter systems, 3rd edition. Kluwer.
  7. Carvalho, A. L., Caldeira, M. V., Santos, S. D. and Duarte, C. B. (2008). Role of the brain-derived neurotrophic factor at glutamatergic synapses. Br J Pharmacol 153 Suppl 1: S310-324.
  8. Caldeira, M. V., Melo, C. V., Pereira, D. B., Carvalho, R. F., Carvalho, A. L. and Duarte, C. B. (2007). BDNF regulates the expression and traffic of NMDA receptors in cultured hippocampal neurons. Mol Cell Neurosci 35(2): 208-219.
  9. Caldeira, M. V., Melo, C. V., Pereira, D. B., Carvalho, R., Correia, S. S., Backos, D. S., Carvalho, A. L., Esteban, J. A. and Duarte, C. B. (2007). Brain-derived neurotrophic factor regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits in hippocampal neurons. J Biol Chem 282(17): 12619-12628.
2 Protocols published
Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for in vitro experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal ...
Biotinylation and Purification of Plasma Membrane-associated Proteins from Rodent Cultured Neurons
Authors:  Margarida Caldeira, Joana S. Ferreira, Ana Luísa Carvalho and Carlos B. Duarte, date: 05/20/2016, view: 2397, Q&A: 0
This protocol aims at the biotin labeling and affinity purification of plasma membrane proteins from cultured neurons. Protein biotinylation consists in the covalent attachment of biotin to proteins. Biotin is a membrane unpermeable molecule with a ...