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Coauthors
Hajime Wada Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Japan
2 protocols

Kumiko Okazaki Joint Research Course for Next Generation Automobile Technology, Graduate School of Science, Hiroshima University, Japan
2 protocols

Shin-ya Miyagishima
  • Department of Cell Genetics, National Institute of Genetics, Japan
Contributions
  • 2 Author merit
2 Protocols published
Preparation of Chloroplast Lipid Membrane and Lipid-Protein Interaction Assay
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 2280, Q&A: 0
Lipid-Protein interaction assay is a method to search lipids, which are bound with proteins in vitro. Since membranes that are spotted with chloroplast lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), ...
Measurement of PI4P Levels in Intact Chloroplasts Isolated from Arabidopsis thaliana
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 2105, Q&A: 0
Phosphatidylinositol 4-phosphate (PI4P), a major species of phosphoinositides, modulates many fundamental cellular processes. We have recently revealed that PI4P plays an important role in chloroplast division as a negative regulator. Despite its ...