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Coauthors
Nienke Buddelmeijer Institut Pasteur, Biology and Genetics of the Bacterial Cell Wall Unit, INSERM Group Avenir, France
2 protocols

Sébastien Gélis-Jeanvoine
  • Micalis INRA, Domaine de Vilvert, France
Contributions
  • 1 Author merit
1 Protocol published
Substituted Cysteine Accessibility Method for Topology and Activity Studies of Membrane Enzymes Forming Thioester Acyl Intermediates in Bacteria
Authors:  Sébastien Gélis-Jeanvoine and Nienke Buddelmeijer, date: 11/05/2015, view: 2334, Q&A: 0
The topology of membrane proteins and enzymes can be determined using various methods including reporter protein fusions and accessibility of cysteine residues to alkylating agents. Here we describe a variation of the substituted cysteine ...