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Coauthors
Leonardo Guilgur Instituto Gulbenkian de Ciencia
2 protocols

Pedro Prudêncio
  • Regenerative Medicine Program, Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, Portugal
  • 2 Author merit

Education

M.S. in Biological Engineering, Universidade do Algarve (FERN) Faro, Portugal, 2010

Current position

Graduate Researcher fellow at Instituto Gulbenkian de Ciencia, Oeiras, Portugal

Publications

  1. Martinho, R. G., Guilgur, L. G. and Prudencio, P. (2015). How gene expression in fast-proliferating cells keeps pace. Bioessays 37(5): 514-524.
  2. Ferreira, T., Prudencio, P. and Martinho, R. G. (2014). Drosophila protein kinase N (Pkn) is a negative regulator of actin-myosin activity during oogenesis. Dev Biol 394(2): 277-291.
  3. Guilgur, L. G., Prudencio, P., Sobral, D., Liszekova, D., Rosa, A. and Martinho, R. G. (2014). Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife 3: e02181.
  4. Alves, E., Henriques, B. J., Rodrigues, J. V., Prudencio, P., Rocha, H., Vilarinho, L., Martinho, R. G. and Gomes, C. M. (2012). Mutations at the flavin binding site of ETF:QO yield a MADD-like severe phenotype in Drosophila. Biochim Biophys Acta 1822(8): 1284-1292.
  5. Guilgur, L. G., Prudencio, P., Ferreira, T., Pimenta-Marques, A. R. and Martinho, R. G. (2012). Drosophila aPKC is required for mitotic spindle orientation during symmetric di-vision of epithelial cells. Development 139(3): 503-513.
2 Protocols published
Protein Extraction from Drosophila Embryos and Ovaries
Authors:  Pedro Prudêncio and Leonardo G. Guilgur, date: 05/05/2015, view: 6487, Q&A: 0
Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos ...
FLP/FRT Induction of Mitotic Recombination in Drosophila Germline
Authors:  Pedro Prudêncio and Leonardo G. Guilgur, date: 05/05/2015, view: 8910, Q&A: 0
The FLP/FRT system is a site-directed recombination technology based on the targeting of a recombination enzyme (flipase - FLP) to specific DNA regions designated as flipase recognition target (FRT) sites. Initially identified in Saccharomyces ...