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Coauthors
Veronica Truniger Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
1 protocol

Miguel Aranda Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
1 protocol

W. Miller Department of Plant Pathology and Microbiology, Iowa State University, USA
1 protocol

Jelena Kraft Department of Plant Pathology and Microbiology, Iowa State University, USA
1 protocol

Raquel N. Sempere
  • Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Spain
Contributions
  • 1 Author merit
1 Protocol published
Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)
This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical ...