Search
Coauthors
John van der Oost Department of Agrotechnology and Food Sciences, Wageningen University, the Netherlands
2 protocols

Daan Swarts Department of Biochemistry, University of Zurich, Switzerland
2 protocols

Matthijs M. Jore
  • Department of Agrotechnology and Food Sciences, Wageningen University, The Netherlands
Contributions
  • 1 Author merit

Education

Ph.D. in Microbiology, Wageningen University, The Netherlands

Current position

Research Associate, Sir William Dunn School of Pathology, University of Oxford, UK

Publications

  1. Swarts, D. C., Jore, M. M., Westra, E. R., Zhu, Y., Janssen, J. H., Snijders, A. P., Wang, Y., Patel, D. J., Berenguer, J., Brouns, S. J. and van der Oost, J. (2014). DNA-guided DNA interference by a prokaryotic Argonaute. Nature 507(7491): 258-261.
  2. Westra, E. R., Swarts, D. C., Staals, R. H., Jore, M. M., Brouns, S. J. and van der Oost, J. (2012). The CRISPRs, they are a-changin': how prokaryotes generate adaptive immunity. Annu Rev Genet 46: 311-339.
  3. van Duijn, E., Barbu, I. M., Barendregt, A., Jore, M. M., Wiedenheft, B., Lundgren, M., Westra, E. R., Brouns, S. J., Doudna, J. A., van der Oost, J. and Heck, A. J. (2012). Native tandem and ion mobility mass spectrometry highlight structural and modular similarities in clustered-regularly-interspaced shot-palindromic-repeats (CRISPR)-associated protein complexes from Escherichia coli and Pseudomonas aeruginosa. Mol Cell Proteomics 11(11): 1430-1441.
  4. Westra, E. R., van Erp, P. B., Kunne, T., Wong, S. P., Staals, R. H., Seegers, C. L., Bollen, S., Jore, M. M., Semenova, E., Severinov, K., de Vos, W. M., Dame, R. T., de Vries, R., Brouns, S. J. and van der Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3. Mol Cell 46(5): 595-605.
  5. Jore, M. M., Brouns, S. J. and van der Oost, J. (2012). RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements. Cold Spring Harb Perspect Biol 4(6).
  6. Wiedenheft, B., Lander, G. C., Zhou, K., Jore, M. M., Brouns, S. J., van der Oost, J., Doudna, J. A. and Nogales, E. (2011). Structures of the RNA-guided surveillance complex from a bacterial immune system. Nature 477(7365): 486-489.
  7. Semenova, E., Jore, M. M., Datsenko, K. A., Semenova, A., Westra, E. R., Wanner, B., van der Oost, J., Brouns, S. J. and Severinov, K. (2011). Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence. Proc Natl Acad Sci U S A 108(25): 10098-10103.
  8. Jore, M. M., Lundgren, M., van Duijn, E., Bultema, J. B., Westra, E. R., Waghmare, S. P., Wiedenheft, B., Pul, U., Wurm, R., Wagner, R., Beijer, M. R., Barendregt, A., Zhou, K., Snijders, A. P., Dickman, M. J., Doudna, J. A., Boekema, E. J., Heck, A. J., van der Oost, J. and Brouns, S. J. (2011). Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Nat Struct Mol Biol 18(5): 529-536.
  9. Westra, E. R., Pul, U., Heidrich, N., Jore, M. M., Lundgren, M., Stratmann, T., Wurm, R., Raine, A., Mescher, M., Van Heereveld, L., Mastop, M., Wagner, E. G., Schnetz, K., Van Der Oost, J., Wagner, R. and Brouns, S. J. (2010). H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO. Mol Microbiol 77(6): 1380-1393.
  10. van der Oost, J., Jore, M. M., Westra, E. R., Lundgren, M. and Brouns, S. J. (2009). CRISPR-based adaptive and heritable immunity in prokaryotes. Trends Biochem Sci 34(8): 401-407.
  11. Brouns, S. J., Jore, M. M., Lundgren, M., Westra, E. R., Slijkhuis, R. J., Snijders, A. P., Dickman, M. J., Makarova, K. S., Koonin, E. V. and van der Oost, J. (2008). Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321(5891): 960-964.
1 Protocol published
Expression and Purification of the Thermus thermophilus Argonaute Protein
Authors:  Daan C. Swarts, Matthijs M. Jore and John van der Oost, date: 10/05/2014, view: 3318, Q&A: 0
The Argonaute protein of Thermus thermophilus (TtAgo) has recently been studied in detail. For its in vitro characterization, TtAgo was purified after heterologous expression in Escherichia coli (E. coli ...