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Coauthors
Paola Chiarugi Department of Biochemical Sciences, Tuscany Tumor Institute and Center for Research, Transfer and High Education DenoTHE, University of Florence, Italy
2 protocols

Tania Fiaschi
  • Department of Biochemical Sciences, Tuscany Tumor Institute and Center for Research, Transfer and High Education DenoTHE, University of Florence, Italy
Contributions
  • 2 Author merit
2 Protocols published
Detection of Released CO2 by Radioactive Lactate
Authors:  Tania Fiaschi and Paola Chiarugi, date: 07/05/2013, view: 3129, Q&A: 0
This method allows to evaluate the degradation of lactate during cellular respiration. During this metabolic process, carbon atoms of lactate can be transformed in carbon dioxide. For this purpose, the radioactive lactate is added to the cells and ...
Analysis of the Incorporation of Carbon Atoms from Radioactive Lactate into Proteins
Authors:  Tania Fiaschi and Paola Chiarugi, date: 07/05/2013, view: 2748, Q&A: 0
This method allows to analyze if the carbon atoms of lactate are embedded into proteins. Indeed, mammalian cells express the transporter of monocarboxylic acids (called MCT1) that allows the entry of lactate into the cell. To this end, cells are ...