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Robert Clubb Department of Chemistry and Biochemistry, UCLA-DOE Institute of Genomics and Proteomics and Molecular Biology Institute, University of California, USA
1 protocol

Megan Sjodt
  • Present address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, USA
Contributions
  • 1 Author merit
1 Protocol published
Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies
Authors:  Megan Sjodt and Robert T. Clubb, date: 04/05/2017, view: 1387, Q&A: 0
Site-specific attachment of paramagnetic spin labels to biomolecules causes distance-dependent line-broadening effects, which can be exploited to study the structure and dynamics of these molecules in solution. This protocol describes how to attach ...