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Coauthors
Thomas Mettenleiter Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Germany
1 protocol

Christina Schröter Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Germany
1 protocol

Melina Vallbracht Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Germany
1 protocol

Barbara G. Klupp
  • Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Germany
  • 1 Author merit

Education

Ph.D, Eberhard-Karls University Tuebingen, Germany, 1992

Current position

Laboratory Head, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler Institut, Greifswald-Insel Riems, Germany

Publications (since 2010)

  1. Hellberg, T., Passvogel, L., Schulz, K. S., Klupp, B. G. and Mettenleiter, T. C. (2016). Nuclear Egress of Herpesviruses: The Prototypic Vesicular Nucleocytoplasmic Transport. Adv Virus Res 94: 81-140.
  2. Zeev-Ben-Mordehai, T., Weberruss, M., Lorenz, M., Cheleski, J., Hellberg, T., Whittle, C., El Omari, K., Vasishtan, D., Dent, K. C., Harlos, K., Franzke, K., Hagen, C., Klupp, B. G., Antonin, W., Mettenleiter, T. C. and Grunewald, K. (2015). Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling. Cell Rep 13(12): 2645-2652.
  3. Hagen, C., Dent, K. C., Zeev-Ben-Mordehai, T., Grange, M., Bosse, J. B., Whittle, C., Klupp, B. G., Siebert, C. A., Vasishtan, D., Bauerlein, F. J., Cheleski, J., Werner, S., Guttmann, P., Rehbein, S., Henzler, K., Demmerle, J., Adler, B., Koszinowski, U., Schermelleh, L., Schneider, G., Enquist, L. W., Plitzko, J. M., Mettenleiter, T. C. and Grunewald, K. (2015). Structural Basis of Vesicle Formation at the Inner Nuclear Membrane. Cell 163(7): 1692-1701.
  4. Schroter, C., Vallbracht, M., Altenschmidt, J., Kargoll, S., Fuchs, W., Klupp, B. G. and Mettenleiter, T. C. (2015). Mutations in Pseudorabies Virus Glycoproteins gB, gD, and gH Functionally Compensate for the Absence of gL. J Virol 90(5): 2264-2272.
  5. Mohl, B. S., Schroter, C., Klupp, B. G., Fuchs, W., Mettenleiter, T. C., Jardetzky, T. S. and Longnecker, R. (2015). Comparative Mutagenesis of Pseudorabies Virus and Epstein-Barr Virus gH Identifies a Structural Determinant within Domain III of gH Required for Surface Expression and Entry Function. J Virol 90(5): 2285-2293.
  6. Bohm, S. W., Backovic, M., Klupp, B. G., Rey, F. A., Mettenleiter, T. C. and Fuchs, W. (2015). Functional Characterization of Glycoprotein H Chimeras Composed of Conserved Domains of the Pseudorabies Virus and Herpes Simplex Virus 1 Homologs. J Virol 90(1): 421-432.
  7. Zuhlsdorf, M., Werten, S., Klupp, B. G., Palm, G. J., Mettenleiter, T. C. and Hinrichs, W. (2015). Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease. PLoS Pathog 11(7): e1005045.
  8. Bohm, S. W., Backovic, M., Klupp, B. G., Rey, F. A., Mettenleiter, T. C. and Fuchs, W. (2015). A replication defect of pseudorabies virus induced by targeted alpha-helix distortion in the syntaxin-like bundle of glycoprotein H (V275P) is corrected by an adjacent compensatory mutation (V271A). J Gen Virol 96(8): 2349-2354.
  9. Schulz, K. S., Klupp, B. G., Granzow, H., Passvogel, L. and Mettenleiter, T. C. (2015). Herpesvirus nuclear egress: Pseudorabies Virus can simultaneously induce nuclear envelope breakdown and exit the nucleus via the envelopment-deenvelopment-pathway. Virus Res 209: 76-86.
  10. Lorenz, M., Vollmer, B., Unsay, J. D., Klupp, B. G., Garcia-Saez, A. J., Mettenleiter, T. C. and Antonin, W. (2015). A single herpesvirus protein can mediate vesicle formation in the nuclear envelope. J Biol Chem 290(11): 6962-6974.
  11. Passvogel, L., Klupp, B. G., Granzow, H., Fuchs, W. and Mettenleiter, T. C. (2015). Functional characterization of nuclear trafficking signals in pseudorabies virus pUL31. J Virol 89(4): 2002-2012.
  12. Bohm, S. W., Eckroth, E., Backovic, M., Klupp, B. G., Rey, F. A., Mettenleiter, T. C. and Fuchs, W. (2015). Structure-based functional analyses of domains II and III of pseudorabies virus glycoprotein H. J Virol 89(2): 1364-1376.
  13. Mahjoub, N., Dhorne-Pollet, S., Fuchs, W., Endale Ahanda, M. L., Lange, E., Klupp, B., Arya, A., Loveland, J. E., Lefevre, F., Mettenleiter, T. C. and Giuffra, E. (2015). A 2.5-kilobase deletion containing a cluster of nine microRNAs in the latency-associated-transcript locus of the pseudorabies virus affects the host response of porcine trigeminal ganglia during established latency. J Virol 89(1): 428-442.
  14. Schroter, C., Klupp, B. G., Fuchs, W., Gerhard, M., Backovic, M., Rey, F. A. and Mettenleiter, T. C. (2014). The highly conserved proline at position 438 in pseudorabies virus gH is important for regulation of membrane fusion. J Virol 88(22): 13064-13072.
  15. Passvogel, L., Janke, U., Klupp, B. G., Granzow, H. and Mettenleiter, T. C. (2014). Identification of conserved amino acids in pUL34 which are critical for function of the pseudorabies virus nuclear egress complex. J Virol 88(11): 6224-6231.
  16. Schulz, K. S., Liu, X., Klupp, B. G., Granzow, H., Cohen, J. I. and Mettenleiter, T. C. (2014). Pseudorabies virus pUL46 induces activation of ERK1/2 and regulates herpesvirus-induced nuclear envelope breakdown. J Virol 88(11): 6003-6011.
  17. Schulz, K. S., Klupp, B. G., Granzow, H. and Mettenleiter, T. C. (2013). Glycoproteins gB and gH are required for syncytium formation but not for herpesvirus-induced nuclear envelope breakdown. J Virol 87(17): 9733-9741.
  18. Passvogel, L., Trube, P., Schuster, F., Klupp, B. G. and Mettenleiter, T. C. (2013). Mapping of sequences in Pseudorabies virus pUL34 that are required for formation and function of the nuclear egress complex. J Virol 87(8): 4475-4485.
  19. Mettenleiter, T. C., Muller, F., Granzow, H. and Klupp, B. G. (2013). The way out: what we know and do not know about herpesvirus nuclear egress. Cell Microbiol 15(2): 170-178.
  20. Maresch, C., Lange, E., Teifke, J. P., Fuchs, W., Klupp, B., Muller, T., Mettenleiter, T. C. and Vahlenkamp, T. W. (2012). Oral immunization of wild boar and domestic pigs with attenuated live vaccine protects against Pseudorabies virus infection. Vet Microbiol 161(1-2): 20-25.
  21. Fuchs, W., Backovic, M., Klupp, B. G., Rey, F. A. and Mettenleiter, T. C. (2012). Structure-based mutational analysis of the highly conserved domain IV of glycoprotein H of pseudorabies virus. J Virol 86(15): 8002-8013.
  22. Hafezi, W., Lorentzen, E. U., Eing, B. R., Muller, M., King, N. J., Klupp, B., Mettenleiter, T. C. and Kuhn, J. E. (2012). Entry of herpes simplex virus type 1 (HSV-1) into the distal axons of trigeminal neurons favors the onset of nonproductive, silent infection. PLoS Pathog 8(5): e1002679.
  23. Grimm, K. S., Klupp, B. G., Granzow, H., Muller, F. M., Fuchs, W. and Mettenleiter, T. C. (2012). Analysis of viral and cellular factors influencing herpesvirus-induced nuclear envelope breakdown. J Virol 86(12): 6512-6521.
  24. Hagen, C., Guttmann, P., Klupp, B., Werner, S., Rehbein, S., Mettenleiter, T. C., Schneider, G. and Grunewald, K. (2012). Correlative VIS-fluorescence and soft X-ray cryo-microscopy/tomography of adherent cells. J Struct Biol 177(2): 193-201.
  25. Schuster, F., Klupp, B. G., Granzow, H. and Mettenleiter, T. C. (2012). Structural determinants for nuclear envelope localization and function of pseudorabies virus pUL34. J Virol 86(4): 2079-2088.
  26. Muller, T., Hahn, E. C., Tottewitz, F., Kramer, M., Klupp, B. G., Mettenleiter, T. C. and Freuling, C. (2011). Pseudorabies virus in wild swine: a global perspective. Arch Virol 156(10): 1691-1705.
  27. Klupp, B. G., Granzow, H. and Mettenleiter, T. C. (2011). Nuclear envelope breakdown can substitute for primary envelopment-mediated nuclear egress of herpesviruses. J Virol 85(16): 8285-8292.
  28. Backovic, M., DuBois, R. M., Cockburn, J. J., Sharff, A. J., Vaney, M. C., Granzow, H., Klupp, B. G., Bricogne, G., Mettenleiter, T. C. and Rey, F. A. (2010). Structure of a core fragment of glycoprotein H from pseudorabies virus in complex with antibody. Proc Natl Acad Sci U S A 107(52): 22635-22640.
  29. Negatsch, A., Granzow, H., Maresch, C., Klupp, B. G., Fuchs, W., Teifke, J. P. and Mettenleiter, T. C. (2010). Ultrastructural analysis of virion formation and intraaxonal transport of herpes simplex virus type 1 in primary rat neurons. J Virol 84(24): 13031-13035.
  30. Kuhn, J., Leege, T., Granzow, H., Fuchs, W., Mettenleiter, T. C. and Klupp, B. G. (2010). Analysis of pseudorabies and herpes simplex virus recombinants simultaneously lacking the pUL17 and pUL25 components of the C-capsid specific component. Virus Res 153(1): 20-28.
  31. Mohl, B. S., Bottcher, S., Granzow, H., Fuchs, W., Klupp, B. G. and Mettenleiter, T. C. (2010). Random transposon-mediated mutagenesis of the essential large tegument protein pUL36 of pseudorabies virus. J Virol 84(16): 8153-8162.
  32. Maresch, C., Granzow, H., Negatsch, A., Klupp, B. G., Fuchs, W., Teifke, J. P. and Mettenleiter, T. C. (2010). Ultrastructural analysis of virion formation and anterograde intraaxonal transport of the alphaherpesvirus pseudorabies virus in primary neurons. J Virol 84(11): 5528-5539.
  33. Muller, T., Klupp, B. G., Freuling, C., Hoffmann, B., Mojcicz, M., Capua, I., Palfi, V., Toma, B., Lutz, W., Ruiz-Fon, F., Gortarzar, C., Hlinak, A., Schaarschmidt, U., Zimmer, K., Conraths, F. J., Hahn, E. C. and Mettenleiter, T. C. (2010). Characterization of pseudorabies virus of wild boar origin from Europe. Epidemiol Infect 138(11): 1590-1600.
1 Protocol published
Transient Transfection-based Fusion Assay for Viral Proteins
Authors:  Melina Vallbracht, Christina Schröter, Barbara G. Klupp and Thomas C. Mettenleiter, date: 03/05/2017, view: 1829, Q&A: 1
Membrane fusion is vital for entry of enveloped viruses into host cells as well as for direct viral cell-to-cell spread. To understand the fusion mechanism in more detail, we use an infection free system whereby fusion can be induced by a minimal ...