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Coauthors
Carmen Bianco Institute of Biosciences and BioResources, Via P. Castellino 111, Italy
1 protocol

Anna Andreozzi Institute of Biosciences and BioResources, Via P. Castellino 111, Italy
1 protocol

Roberto Defez
  • Institute of Biosciences and BioResources, Via P. Castellino 111, Italy
  • 1 Author merit

Education

Degree, Università Federico II di Napoli, Italy, 1980

Current position

Researcher, IBBR, CNR, Italy

Publications (since 2000)

  1. Defez, R., Esposito, R., Angelini, C. and Bianco, C. (2016). Overproduction of Indole-3-Acetic Acid in Free-Living Rhizobia Induces Transcriptional Changes Resembling Those Occurring in Nodule Bacteroids. Mol Plant Microbe Interact 29(6): 484-495.
  2. Defez, R. (2016). Deforming mirrors converting GMOs into smog. Emerald Insight, Food and Nutrition 46(5): 620-627.
  3. Mita, L., Grumiro, L., Rossi, S., Bianco, C., Defez, R., Gallo, P., Mita, D. G. and Diano, N. (2015). Bisphenol A removal by a Pseudomonas aeruginosa immobilized on granular activated carbon and operating in a fluidized bed reactor. J Hazard Mater 291: 129-135.
  4. Germanà, M. A., Micheli, M., Chiancone, B., Bianco, C., Casales, F. G. and Defez, R. (2015). Biotization of encapsulated vitro-derived propagules of Carrizo citrange. Article in Acta horticulturae.
  5. Bianco, C., Senatore, B., Arbucci, S., Pieraccini, G. and Defez, R. (2014). Modulation of endogenous indole-3-acetic acid biosynthesis in bacteroids within Medicago sativa nodules. Appl Environ Microbiol 80(14): 4286-4293.
  6. Bianco, C. and Defez, R. (2011). Soil Bacteria Support and Protect Plants Against Abiotic Stresses. In: Shanker, A. and Venkateswarlu, B. (eds.). Abiotic Stress in Plants - Mechanisms and Adaptations.
  7. Bianco, C., Rotino, G. L., Campion, B., Anas, I. and Defez, R. (2010). How to improve legume production under severe environmental stresses. Journal of Biotechnology 150: 119-119.
  8. Bianco, C. and Defez, R. (2010). Auxins upregulate nif and fix genes. Plant Signal Behav 5(10): 1290-1294.
  9. Bianco, C. and Defez, R. (2010). Improvement of phosphate solubilization and Medicago plant yield by an indole-3-acetic acid-overproducing strain of Sinorhizobium meliloti. Appl Environ Microbiol 76: 4626-4632.
  10. Bianco, C., Imperlini, E. and Defez, R. (2009). Legumes like more IAA. Plant Signal Behav 4(8): 763-765.
  11. Bianco, C. and Defez, R. (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain. J Exp Bot 60(11): 3097-3107.
  12. Imperlini, E., Bianco, C., Lonardo, E., Camerini, S., Cermola, M., Moschetti, G. and Defez, R. (2009). Effects of indole-3-acetic acid on Sinorhizobium meliloti survival and on symbiotic nitrogen fixation and stem dry weight production. Appl Microbiol Biotechnol 83(4): 727-738.
  13. Lantin, C., Bulawa, B., Haselier, A., Defez, R. and Priefer, U. B. (2008). Effect of a Genetically Modified Rhizobium leguminosarum Strain on Bacterial and Fungal Diversity in the Rhizosphere of Pisum sativum. Current Plant Science and Biotechnology in Agriculture 42: 65-66.
  14. Camerini, S., Senatore, B., Lonardo, E., Imperlini, E., Bianco, C., Moschetti, G., Rotino, G. L., Campion, B. and Defez, R. (2008). Introduction of a novel pathway for IAA biosynthesis to rhizobia alters vetch root nodule development. Arch Microbiol 190(1): 67-77.
  15. Bianco, C., Imperlini, E., Calogero, R., Senatore, B., Amoresano, A., Carpentieri, A., Pucci, P. and Defez, R. (2006). Indole-3-acetic acid improves Escherichia coli's defences to stress. Arch Microbiol 185(5): 373-382.
  16. Moschetti, G., Peluso, A., Protopapa, A., Anastasio, M., Pepe, O. and Defez, R. (2005). Use of nodulation pattern, stress tolerance, nodC gene amplification, RAPD-PCR and RFLP-16S rDNA analysis to discriminate genotypes of Rhizobium leguminosarum biovar viciae. Syst Appl Microbiol 28(7): 619-631.
  17. Pandolfini, T., Rotino, G. L., Camerini, S., Defez, R. and Spena, A. (2002). Optimisation of transgene action at the post-transcriptional level: high quality parthenocarpic fruits in industrial tomatoes. BMC Biotechnology 2: 1.
  18. Priefer, U. B., Aurag, J., Boesten, B., Bouhmouch, I., Defez, R., Filali-Maltouf, A., Miklis, M., Moawad, H., Mouhsine, B., Prell, J., Schluter, A. and Senatore, B. (2001). Characterisation of Phaseolus symbionts isolated from Mediterranean soils and analysis of genetic factors related to pH tolerance. J Biotechnol 91(2-3): 223-236.
  19. Schluster, A., Noshlen, M., Krasmer, M., Defez, R. and Priefer, U. B. (2000). The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/ uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation. Microbiology 146: 2987–2996.
  20. Pandolfini, T., Storlazzi, A., Calabria, E., Defez, R. and Spena, A. (2000). The spliceosomal intron of the rolA gene of agrobacterium rhizogenes is a prokaryotic promoter. Mol Microbiol 35(6): 1326-1334.
1 Protocol published
Quantification of Triphenyl-2H-tetrazoliumchloride Reduction Activity in Bacterial Cells
Authors:  Roberto Defez, Anna Andreozzi and Carmen Bianco, date: 01/20/2017, view: 1880, Q&A: 0
This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC ...