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Coauthors
Yvonne Neldner Department of Biochemistry, University of Zurich, Switzerland
1 protocol

Farooque Shaik Laboratory of Biomolecular Research, Paul Scherrer Institute, Switzerland
1 protocol

Yung-Ning Chang Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Germany
1 protocol

Eric R. Geertsma
  • Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Germany
  • 1 Author merit

Education

Ph.D in Membrane Biochemistry, Department of Biochemistry, University of Groningen, Netherlands, 2005

Current position

Professor in Membrane Biochemistry, Institute for Biochemistry, Goethe University Frankfurt, Frankfurt am Main, Germany

Publications (since 2008)

  1. Chang, Y. N. and Geertsma, E. R. (2017). The novel class of seven transmembrane segment inverted repeat carriers. Biol Chem 398(2): 165-174.
  2. Geertsma, E. R., Chang, Y. N., Shaik, F. R., Neldner, Y., Pardon, E., Steyaert, J. and Dutzler, R. (2016). Corrigendum: Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family. Nat Struct Mol Biol 23(5): 462.
  3. Geertsma, E. R., Chang, Y. N., Shaik, F. R., Neldner, Y., Pardon, E., Steyaert, J. and Dutzler, R. (2015). Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family. Nat Struct Mol Biol 22(10): 803-808.
  4. Bukowska, M. A., Hohl, M., Geertsma, E. R., Hurlimann, L. M., Grutter, M. G. and Seeger, M. A. (2015). A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter. Biochemistry 54(19): 3086-3099.
  5. Marino, J., Hohl, M., Seeger, M. A., Zerbe, O. and Geertsma, E. R. (2015). Bicistronic mRNAs to enhance membrane protein overexpression. J Mol Biol 427(4): 943-954.
  6. Ehrnstorfer, I. A., Geertsma, E. R., Pardon, E., Steyaert, J. and Dutzler, R. (2014). Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport. Nat Struct Mol Biol 21(11): 990-996.
  7. Geertsma, E. R. (2014). FX cloning: a simple and robust high-throughput cloning method for protein expression. Methods Mol Biol 1116: 153-164.
  8. Geertsma, E. R. (2013). FX cloning: a versatile high-throughput cloning system for characterization of enzyme variants. Methods Mol Biol 978: 133-148.
  9. Marino, J., Geertsma, E. R. and Zerbe, O. (2012). Topogenesis of heterologously expressed fragments of the human Y4 GPCR. Biochim Biophys Acta 1818(12): 3055-3063.
  10. Malojcic, G., Geertsma, E. R., Brozzo, M. S. and Glockshuber, R. (2012). Mechanism of the prokaryotic transmembrane disulfide reduction pathway and its in vitro reconstitution from purified components. Angew Chem Int Ed Engl 51(28): 6900-6903.
  11. Marreddy, R. K., Pinto, J. P., Wolters, J. C., Geertsma, E. R., Fusetti, F., Permentier, H. P., Kuipers, O. P., Kok, J. and Poolman, B. (2011). The response of Lactococcus lactis to membrane protein production. PLoS One 6(8): e24060.
  12. Geertsma, E. R. and Dutzler, R. (2011). A versatile and efficient high-throughput cloning tool for structural biology. Biochemistry 50(15): 3272-3278.
  13. Linares, D. M., Geertsma, E. R. and Poolman, B. (2010). Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins. J Mol Biol 401(1): 45-55.
  14. Marreddy, R. K., Geertsma, E. R., Permentier, H. P., Pinto, J. P., Kok, J. and Poolman, B. (2010). Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis. PLoS One 5(4): e10317.
  15. Geertsma, E. R. and Poolman, B. (2010). Production of membrane proteins in Escherichia coli and Lactococcus lactis. Methods Mol Biol 601: 17-38.
  16. Kuiper, J. M., Pluta, R., Huibers, W. H., Fusetti, F., Geertsma, E. R. and Poolman, B. (2009). A method for site-specific labeling of multiple protein thiols. Protein Sci 18(5): 1033-1041.
  17. Mulligan, C., Geertsma, E. R., Severi, E., Kelly, D. J., Poolman, B. and Thomas, G. H. (2009). The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter. Proc Natl Acad Sci U S A 106(6): 1778-1783.
  18. Geertsma, E. R., Groeneveld, M., Slotboom, D. J. and Poolman, B. (2008). Quality control of overexpressed membrane proteins. Proc Natl Acad Sci U S A 105(15): 5722-5727.
  19. Geertsma, E. R., Nik Mahmood, N. A., Schuurman-Wolters, G. K. and Poolman, B. (2008). Membrane reconstitution of ABC transporters and assays of translocator function. Nat Protoc 3(2): 256-266.
1 Protocol published
Production, Purification and Crystallization of a Prokaryotic SLC26 Homolog for Structural Studies
Authors:  Yung-Ning Chang, Farooque R. Shaik, Yvonne Neldner and Eric R. Geertsma, date: 02/05/2017, view: 1746, Q&A: 0
The SLC26 or SulP proteins constitute a large family of anion transporters that are ubiquitously expressed in pro- and eukaryotes. In human, SLC26 proteins perform important roles in ion homeostasis and malfunctioning of selected members is ...